Tie-1 Antibody (8D12B10) - BSA Free

Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.

Immunohistochemistry: Tie-1 Antibody (8D12B10) [NBP2-37270] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TIE1 mouse mAb with DAB staining.

Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against human TIE1 (AA: 385-607) recombinant protein. (Expected MW is 50.6 kDa)

Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against HEK293 (1) and TIE1 (AA: 385-607)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Tie-1 Antibody (8D12B10) [NBP2-37270] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 8D12B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Tie-1 Antibody (8D12B10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Tie-1 (AA: 385-607) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIE1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Tie-1 Antibody (8D12B10) - BSA Free

• EC 2.7.10
• EC 2.7.10.1
• JTK14soluble TIE1 variant 3
• receptor tyrosine kinase
• soluble TIE1 variant 1
• soluble TIE1 variant 2
• soluble TIE1 variant 5
• Tie1
• Tie-1
• TIEsoluble TIE1 variant 4
• tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
• tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• tyrosine-protein kinase receptor Tie-1

Background

TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIE1