Tie-2 Antibody (AP-MAB0807)

Product Discontinued

Tie-2 Antibody (AP-MAB0807) Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse Tie2 extracellular domain).
• Specificity: This is specific for the mouse Tie2 extracellular domain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TEK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:100-1:400
• Application Notes: ICC/IF reactivity reported in scientific literature (PMID:21334399)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Tie-2 Antibody (AP-MAB0807)

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (NB110-85475)(1)

Publication using NB110-85475

• Fernandez-Ruiz V, Kawa M, Berasain C, Iniguez M, Schmitz V, Martinez-Anso E, Inarrairaegui M, Herrero I, Sangro B, D'Avola D, Quiroga J, Qian C, Prieto J. Treatment of murine fulminant hepatitis with genetically engineered endothelial progenitor cells. J Hepatol;55(4):828. 2011-10-01 [PMID: 21334399] (ICC/IF, IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: TEK
• Entrez:
  • 21687(Mouse)
• Uniprot:
  • Q02858(Mouse)