TIMP-1 Antibody (4D12)

Western Blot: TIMP-1 Antibody (4D12) [H00007076-M01] - Analysis of TIMP1 expression in transfected 293T cell line by TIMP1 monoclonal antibody (M01), clone 4D12.Lane 1: TIMP1 transfected lysate(23.2 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: TIMP-1 Antibody (4D12) [H00007076-M01] - Analysis of TIMP1 transfected lysate using anti-TIMP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TIMP1 MaxPab rabbit polyclonal antibody.

ELISA: TIMP-1 Antibody (4D12) [H00007076-M01] - Detection limit for recombinant GST tagged TIMP1 is approximately 3ng/ml as a capture antibody.

Product Discontinued

TIMP-1 Antibody (4D12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TIMP1 (AAH07097, 1 a.a. ~ 169 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPFEPLASGILLLLWLIAPSRACTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYKGFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCL
• Specificity: TIMP1 - TIMP metallopeptidase inhibitor 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TIMP-1 Antibody (4D12)

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMP1
• Entrez:
  • 7076(Human)
• OMIM:
  • 305370(Human)