TIMP-2 Antibody (OTI1A6)

Western Blot: TIMP2 Antibody (1A6) [NBP2-01573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIMP2.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human pancreas tissue using anti-TIMP2 mouse monoclonal antibody.

Flow Cytometry: TIMP2 Antibody (1A6) [NBP2-01573] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TIMP2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TIMP2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TIMP2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIMP2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TIMP2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human breast tissue using anti-TIMP2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human endometrium tissue using anti-TIMP2 mouse monoclonal antibody.

Flow Cytometry: TIMP2 Antibody (1A6) [NBP2-01573] - Analysis of Jurkat cells, using anti-TIMP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TIMP-2 Antibody (OTI1A6) Summary

• Immunogen: Full length human recombinant protein of human TIMP2(NP_003246) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 21.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIMP-2 Antibody (OTI1A6)

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-2 Antibody (NBP2-01573)(3)

Publications using NBP2-01573

• Helmin-Basa A, Kubiszewska I, Wiese-Szadkowska M et al. Expression of Matrix Metalloproteinases in the Circulating Immune Cells in Children with Helicobacter pylori Infection—Correlation with Clinical Factors International Journal of Molecular Sciences 2023-10-27 [PMID: 37958643] (Flow Cytometry, Human)
• Premadasa LS, Lee E, McDew-White M et al. Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection JCI insight 2023-04-10 [PMID: 37036007] (ICC/IF)
• Kollaras V, Valsami G, Lambropoulou M et al. Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model Acta Cirúrgica Brasileira 2021-11-08 [PMID: 34755764] (, Immunohistochemistry)

Bioinformatics

• Gene Symbol: TIMP2
• Entrez:
  • 7077(Human)