TLR (Cell Surface) Screening Antibody Pack

Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - TLR1 and TLR2 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563] and TLR2 Antibody (TL2.1) [NB100-56722] .

Dual RNAscope ISH-IHC: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, catalog #311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog #NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Western Blot: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR2 Antibody (TL2.1) [NB100-56722]

Immunohistochemistry-Paraffin: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]. TLR5 Antibody (19D759.2) [NBP2-24787]

Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Detection of TLR10 on human PBMC using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody. Analysis using the Alexa Fluor (R) 647 conjugate of TLR10 antibody [NBP1-70343].

Immunohistochemistry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/33/1/45), licensed under a CC-BY license. TLR4 Antibody (76B357.1) [NB100-56566]

• Reactivity: Hu, Mu
• Applications: WB, DB, Flow, ICC/IF, IHC

TLR (Cell Surface) Screening Antibody Pack Summary

• Immunogen: See individual datasheets.

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for TLR (Cell Surface) Screening Antibody Pack (NBP2-25086)(1)

Publication using NBP2-25086

• Ferrandez E, Gutierrez O, Segundo DS, Fernandez-Luna JL. NFkB activation in differentiating glioblastoma stem-like cells is promoted by hyaluronic acid signaling through TLR4 Sci Rep 2018-04-20 [PMID: 29679017] (FLOW, Human)