TLR Downstream Antibody Pack

Western Blot: TLR Downstream Antibody Pack [NBP2-25084] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. TLR4 Antibody [NBP2-24538] and MD-2 Antibody [NB100-56655]

Western Blot: TLR Downstream Antibody Pack [NBP2-25084] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity.Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY license. MyD88 Antibody [NB100-56698]

Immunohistochemistry: TLR Downstream Antibody Pack [NBP2-25084] - Analysis of human spleen using MD-1 antibody [NB100-56700] at 10 ug/ml.

TLR Downstream Antibody Pack [NBP2-25084] - Analysis of IRAK-1 in 1) human HeLa and 2) mouse NIH3T3 lysate using IRAK-1 antibody [NB100-56699] at 1 ug/ml.

Product Discontinued

TLR Downstream Antibody Pack Summary

• Immunogen: See individual datasheets.
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: See individual datasheets for validated applications.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Purity: Protein G purified

Notes

This pack contains 1 vial each of: NB100-56700SS (0.025 mg), NB100-56712SS (0.025 mg), NB100-56447SS (0.025 mg), NB100-56655SS (0.025 mg), NB100-56699SS (0.025 mg), NB100-56698SS (0.025 mg), NB500-597-0.05mg (0.05 mg).

Alternate Names for TLR Downstream Antibody Pack

• Toll like receptors
• Toll-like receptors

Background

Detection of PAMPs by TLRs represents the immune recognition step that accounts for self-nonself discrimination, triggers the innate immune response, and act as efficient molecules for priming and optimizing antigen specific adaptive host response. The TLR gene family and their pathways have been evolutionarily well conserved in both invertebrates and vertebrates. Among the various molecules involved in the successful coordination of the TLR pathway the most important is the adapter protein MyD88 that acts via IRAK1, IRAK2 and TRAF6. Stimulation of MyD88 leads to association with the IL-1R associated kinases, IRAK1, IRAK2 and IRAK4, where IRAK4 is regarded as the central mediator of TLR signaling which acts by activating IRAK1. Activation by these molecules leads to phosphorylation and degradation of IkB leading to the consequent release of NF-kB (p65) and NF-kB (p50). Thus stimulation of TLR by its cognate ligands leads to activation of the NF-kappa-B pathway, cytokine secretion and inflammatory response. TLR4 signaling may require additional molecules like MD-1, important for efficient CD180 cell surface expression and MD-2 to respond to LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.