TOR/mTOR Antibody

Product Discontinued

TOR/mTOR Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 2430-2480 of Human mTOR.
• Specificity: mTOR (S2442) pAb detects endogenous levels of mTOR protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of mTOR (S2442) pAb in extracts from HUVEC cells.
  Immunohistochemistry (IHC) analyzes of mTOR (S2442) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 289 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOR/mTOR Antibody

• EC 2.7.11.1
• FK506 binding protein 12-rapamycin associated protein 1
• FK506 binding protein 12-rapamycin associated protein 2
• FK506-binding protein 12-rapamycin complex-associated protein 1
• FKBP12-rapamycin complex-associated protein
• FLJ44809
• FRAP
• FRAP1
• FRAP1FKBP12-rapamycin complex-associated protein 1
• FRAP2
• Mammalian target of rapamycin
• mechanistic target of rapamycin (serine/threonine kinase)
• Mechanistic target of rapamycin
• MTOR
• RAFT1
• rapamycin and FKBP12 target 1
• rapamycin associated protein FRAP2
• Rapamycin target protein 1
• RAPT1FKBP-rapamycin associated protein
• serine/threonine-protein kinase mTOR
• TOR

Background

Regulatory associated protein of mTOR, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (mTOR), and is essential for mTOR signalling in vivo. Raptor binding to mTOR is critical for mTORcatalysed substrate phosphorylation of 4EBP1. The Raptor-mTOR complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of mTOR kinase activity under nutrient-deprived conditions and is an important component in the mTOR pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOR/mTOR Antibody (NBP1-19855)(3)

Publications using NBP1-19855

• Zhang S, Cao F, Li W, Abumaria N TRPM7 kinase activity induces amyloid-? degradation to reverse synaptic and cognitive deficits in mouse models of Alzheimer's disease Science signaling 2023-07-11 [PMID: 37433006] (WB, Mouse)
• Cameron AJM, Veeriah S, Marshall JJT et al. Uncoupling TORC2 from AGC kinases inhibits tumour growth Oncotarget. 2017-10-17 [PMID: 29156676] (Human)
• Bennett HL, Stockley J, Fleming JT et al. Does androgen-ablation therapy (AAT) associated autophagy have a pro-survival effect in LNCaP human prostate cancer cells? BJU Int 2012-08-16 [PMID: 22897391] (WB, Human)

Review for TOR/mTOR Antibody (NBP1-19855) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Varkha Agrawal WB Mouse 07/14/2014

Summary

• Application: Western Blot
• Sample Tested: mouse placenta and uterus
• Species: Mouse
• Lot: 360939

Bioinformatics

• Gene Symbol: MTOR
• Entrez:
  • 2475(Human)
  • 56717(Mouse)
  • 56718(Rat)
• Uniprot:
  • P42345()