TPT1/TCTP Antibody (3C7) - Azide and BSA Free

Western Blot: TPT1/TCTP Antibody (3C7) [H00007178-M01] - TPT1 monoclonal antibody (M01), clone 3C7 Analysis of TPT1 expression in HepG2.

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (3C7) [H00007178-M01] - Analysis of monoclonal antibody to TPT1 on HeLa cell. Antibody concentration 30 ug/ml.

ELISA: TPT1/TCTP Antibody (3C7) [H00007178-M01] - Detection limit for recombinant GST tagged TPT1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TPT1/TCTP Antibody (3C7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPT1 (AAH22436, 35 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC
• Specificity: TPT1 - tumor protein, translationally-controlled 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TPT1/TCTP Antibody (3C7) - Azide and BSA Free

• FLJ27337
• Fortilin
• Histamine-releasing factor
• HRF
• p02
• p23
• TCTP
• TCTPHRFfortilin
• TPT1
• translationally-controlled tumor protein
• tumor protein, translationally-controlled 1

Background

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPT1/TCTP Antibody (H00007178-M01)(2)

Publications using H00007178-M01

• Chen Ke, Huang Chunhua, Yuan Jia et al. Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. Mol Biol Evol. 2014-06-02 [PMID: 24890374]
• Chen K, Chen S, Huang C et al. TCTP increases stability of hypoxia-inducible factor 1alpha by interaction with and degradation of the tumour suppressor VHL. Biol Cell. 2013-05-01 [PMID: 23387829] (Human)

Bioinformatics

• Gene Symbol: TPT1
• Entrez:
  • 7178(Human)
• OMIM:
  • 600763(Human)
• Uniprot:
  • AAH22436(Human)