Human Transferrin ELISA Kit (Colorimetric) Summary
Description |
TF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TF in cell culture supernatant and urine. Suitable Sample and Volume: Cell Culture Samples, CSF, Milk, Saliva, Urine, 50 uL |
Standard Curve Range |
1.56 to 100 ng/mL |
Sensitivity |
1.5 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 9.6% |
Intra-Assay |
CV% < 4.9% |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
TF |
Applications/Dilutions
Dilutions |
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Application Notes |
TF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TF in cell culture supernatant and urine. |
Publications |
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Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Transferrin Antibody (50x): A 50-fold concentrated biotinylated polyclonalantibody against transferring.
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
- Human Transferrin Microplate: A microplate coated with a polyclonal antibody againsthuman Transferrin.
- Human Transferrin Standard: Human Transferrin in a buffered protein base(lyophilized).
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
- Sealing Tapes: Pre-cut, pressure-sensitive sealing tapes that can be cut to fit the formatof the individual assay.
- Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Transferrin ELISA Kit (Colorimetric)
Background
The TF (Human) ELISA Kit is designed for detection of human transferrin in urine, milk, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures transferrin in less than 4 hours. A polyclonal antibody specific for transferrin has been pre-coated onto a 96-well microplate with removable strips. Transferrin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for transferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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