Human Transferrin ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0510
    • Availability
      Product Discontinued

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Human Transferrin ELISA Kit (Colorimetric) Summary

Description
TF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TF in cell culture supernatant and urine.
Suitable Sample and Volume: Cell Culture Samples, CSF, Milk, Saliva, Urine, 50 uL
Standard Curve Range
1.56 to 100 ng/mL
Sensitivity
1.5 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 9.6%
Intra-Assay
CV% < 4.9%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
TF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TF in cell culture supernatant and urine.
Publications
Read Publication using KA0510.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Transferrin Antibody (50x): A 50-fold concentrated biotinylated polyclonalantibody against transferring.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. Human Transferrin Microplate: A microplate coated with a polyclonal antibody againsthuman Transferrin.
  4. Human Transferrin Standard: Human Transferrin in a buffered protein base(lyophilized).
  5. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  6. Sealing Tapes: Pre-cut, pressure-sensitive sealing tapes that can be cut to fit the formatof the individual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Transferrin ELISA Kit (Colorimetric)

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

The TF (Human) ELISA Kit is designed for detection of human transferrin in urine, milk, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures transferrin in less than 4 hours. A polyclonal antibody specific for transferrin has been pre-coated onto a 96-well microplate with removable strips. Transferrin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for transferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Transferrin ELISA Kit (KA0510)(1)

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Additional Transferrin Products

Research Areas for Transferrin ELISA Kit (KA0510)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF