Trastuzumab Antibody (2261E) [Unconjugated]

Western blot of Centuximab (negative control), Rituximab (negative control), Trastuzumab, and Teprotumumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Trastuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB95471) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Trastuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

MCF-7 Human cell line was stained with Human Trastuzumab (anti-Human ErbB2/Her2) Monoclonal Antibody (MAB9589), then Anti-Trastuzumab (Anti-Idiotype) (Catalog # MAB95471, filled histogram) or Rabbit IgG Isotype Control Antibody (MAB1050, open histogram) followed by APC-conjugated anti-rabbit IgG secondary antibody (F0111).

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Trastuzumab (Anti-Idiotype) Antibody (Catalog # MAB95471, 333 ng/mL) detected Trastuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

• Reactivity: All-Multi
• Applications: WB, ELISA, Flow
• Clone: 2261E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Trastuzumab Antibody (2261E) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Full-length Trastuzumab
• Specificity: Detects Trastuzumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trastuzumab Antibody (2261E) [Unconjugated]

• Trastuzumab

Background

The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trastuzumab Antibody (MAB95471)(5)

Publications using MAB95471

• Jason T. Smith, Alena Rudkouskaya, Shan Gao, Juhi M. Gupta, Arin Ulku, Claudio Bruschini et al. In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera Optica 8/15/2022 [PMID: 35968259]
• Jason T. Smith, Alena Rudkouskaya, Shan Gao, Juhi M. Gupta, Arin Ulku, Claudio Bruschini et al. In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera Optica 2022-08-15 [PMID: 35968259] (Immunohistochemistry, Mouse)
• Marien Ochoa, Alena Rudkouskaya, Jason T. Smith, Xavier Intes, Margarida Barroso Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug–Target Engagement Methods in Molecular Biology 3/16/2022 [PMID: 35094361]
• 2020-12-17;0(0):. 2020-12-17 [PMID: 33348564] (Western Blot, Human)
• 2019-01-23;0(0):. 2019-01-23 [PMID: 30674653] (IHC, Mouse)