TREM2 Antibody (263602) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 263602
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TREM2 Antibody (263602) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TREM2
  His19-Ser174
  Accession # Q9NZC2
• Specificity: Detects human TREM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TREM1, recombinant mouse (rm) TREM2b, or rmTREM3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TREM2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM2 Antibody (263602) [Unconjugated]

• PLOSL2
• TREM2
• TREM-2
• Trem2a
• Trem2b
• Trem2c
• TREM-2triggering receptor expressed on myeloid cells 2a
• Triggering receptor expressed on monocytes 2
• triggering receptor expressed on myeloid cells 2

Background

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily . Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM2 Antibody (MAB1828)(7)

Publications using MAB1828

• Subramani, M;Lambrecht, B;Ahmad, I; Human microglia-derived proinflammatory cytokines facilitate human retinal ganglion cell development and regeneration Stem cell reports 2024-07-22 [PMID: 39059376] (Immunocytochemistry, Human)
• Filipello F, You SF, Mirfakhar FS et al. Defects in lysosomal function and lipid metabolism in human microglia harboring a TREM2 loss of function mutation Acta Neuropathologica 2023-06-01 [PMID: 37115208]
• Rumana Akhter, Yvonne Shao, Shane Formica, Maria Khrestian, Lynn M. Bekris TREM2 alters the phagocytic, apoptotic and inflammatory response to A beta 42 in HMC3 cells Molecular Immunology 2021-03-01 [PMID: 33461764]
• Grace E. Weber, Katherine A. Koenig, Maria Khrestian, Yvonne Shao, Elizabeth D. Tuason, Marie Gramm et al. An Altered Relationship between Soluble TREM2 and Inflammatory Markers in Young Adults with Down Syndrome: A Preliminary Report The Journal of Immunology 2020-03-01 [PMID: 31959733]
• J Cooper-Kno, C Green, G Altschuler, W Wei, JJ Bury, PR Heath, M Wyles, C Gelsthorpe, JR Highley, A Lorente-Po, T Beck, K Doyle, K Otero, B Traynor, J Kirby, PJ Shaw, W Hide A data-driven approach links microglia to pathology and prognosis in amyotrophic lateral sclerosis Acta Neuropathol Commun, 2017-03-16;5(1):23. 2017-03-16 [PMID: 28302159] (ELISA Development (Capture), Human)
• Quan DN, Cooper MD, Potter JL, Roberts MH, Cheng H, Jarvis GA TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells. Mucosal Immunol, 2008-03-05;1(3):229-38. 2008-03-05 [PMID: 19079182] (Flow Cytometry, Human)
• Ochi S, Shinohara M, Sato K, Gober HJ, Koga T, Kodama T, Takai T, Miyasaka N, Takayanagi H Pathological role of osteoclast costimulation in arthritis-induced bone loss. Proc. Natl. Acad. Sci. U.S.A., 2007-06-25;104(27):11394-9. 2007-06-25 [PMID: 17592115] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TREM2
• Uniprot:
  • Q9NZC2()