TrkC Antibody (2B7)

Product Discontinued

TrkC Antibody (2B7) Summary

• Immunogen: Immunogen was a linear recombinant human TrkC peptide conjugated to KLH.
• Specificity: The is competitively blocked by the natural TrkC ligand, Neurotrophin 3 (NT3) but not the homologue Nerve Growth Factor (NGF) in a dose dependent manner.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTRK3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Uses include Flow Cytometry, Western Blot(non-reducing conditions), immunohistochemistry-paraffin and biological function studies (eg, activation of TrkC without p75).
• Theoretical MW: 94.43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Rat and Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water.

Alternate Names for TrkC Antibody (2B7)

• EC 2.7.10
• EC 2.7.10.1
• ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• ETV6-NTRK3 fusion
• gp145(trkC)
• GP145-TrkC
• Neurotrophic tyrosine kinase receptor type 3
• neurotrophic tyrosine kinase, receptor, type 3
• NT 3 receptor
• NT-3 growth factor receptor
• NTRK3
• TrkC tyrosine kinase
• TrkC
• trk-C
• TRKCneurotrophin 3 receptor
• tyrosine kinase receptor C

Background

TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers. The mAb 2B7 has been well-characterized as a selective TrkC ligand that can be used to target the human and murine TrkC receptor without p75NTR binding and activation. The antibody acts as a partial agonist to the TrkC receptor and defines a new "hot-spot" within the juxtamembrane region of the extracellular ectodomain of the receptor. Binding to this hot spot, the receptor induces survival, but not differentiation of TrkC expressing cells. Activation of the TrkC receptor is accompanied by activation of the signalling molecules, Akt and MAPK. This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkC Antibody (M-958-100)(4)

Publications using M-958-100

• Guillemard V et al. (2010) An agonistic mAb directed to the TrkC receptor juxtamembrane region defines a trophic hot spot and interactions with p75 coreceptors. [PMID: 19953569]
• Baker KA et al. (2007) Dorsal column sensory axons lack TrkC and are not rescued by local neurotrophin-3 infusions following spinal cord contusion in adult rats. [PMID: 17316612]
• Esteban PF et al. (2006) A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin. [PMID: 16636148]
• Zaccaro M et al. (2005) Chemistry and Biology 12. pp. 1015-1028. 2005-01-01 [PMID: 16183026]

Bioinformatics

• Gene Symbol: NTRK3
• Entrez:
  • 4916(Human)
  • 18213(Mouse)
  • 29613(Rat)
• Uniprot:
  • Q16288()