Twist-1 Antibody (10E4E6) - BSA Free

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody at 1:100 dilution. IHC-P image submitted by a verified customer review.

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. Expected MW is 31.9 kDa.

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.

Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.

ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, KD
• Clone: 10E4E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Twist-1 Antibody (10E4E6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Twist-1 (AA: 9-74) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TWIST1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Knockdown Validated
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is CyTOF ready. Use in Knockdown Validated reported in scientific literature (PMID: 31113881).
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Twist-1 Antibody (10E4E6) - BSA Free

• acrocephalosyndactyly 3
• ACS3
• B-HLH DNA binding protein
• BHLHA38
• bHLHa38BPES3
• BPES3
• Class A basic helix-loop-helix protein 38
• CRS1
• H-twist
• H-twistblepharophimosis, epicanthus inversus and ptosis 3
• SCSBPES2
• twist homolog 1 (Drosophila)
• TWIST homolog of drosophila
• TWIST
• Twist1
• Twist-1
• twist-related protein 1

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Twist-1 Antibody (NBP2-37364)(11)

Publications using NBP2-37364

• Seok Han B, Ko S, Seok Park M et Al. Lidocaine combined with general anesthetics impedes metastasis of breast cancer cells via inhibition of TGF-?/Smad-mediated EMT signaling by reprogramming tumor-associated macrophages Int Immunopharmacol 2024-09-22 [PMID: 39312860]
• Pu, T;Wang, J;Wei, J;Zeng, A;Zhang, J;Chen, J;Yin, L;Li, J;Lin, TP;Melamed, J;Corey, E;Gao, AC;Wu, BJ; Stromal-derived MAOB promotes prostate cancer growth and progression Science advances 2024-02-09 [PMID: 38335292]
• Debarpan Mitra, Depanwita Saha, Gaurav Das, Rimi Mukherjee, Samir Banerjee, Neyaz Alam, Saunak Mitra Mustafi, Partha Nath, Anuj Majumder, Biswanath Majumder, Nabendu Murmu Lupeol synergizes with 5-fluorouracil to combat c-MET/EphA2 mediated chemoresistance in triple negative breast cancer iScience 2023-11-04 [PMID: 38047085]
• Morris HT, Bamlet WR, Razidlo GL, Machesky LM FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma Pathology, research and practice 2023-10-02 [PMID: 37832352] (IHC, Human)
  
  Details:
  Dilution 1:400
• Chakraborty A, Halder B, Mondal S et al. NADPH oxidase 1 in chronic pancreatitis-activated pancreatic stellate cells facilitates the progression of pancreatic cancer e-century.us 2023-01-30 [PMID: 36777508]
• Han BS, Jung KH, Lee JE et al. Lidocaine enhances the efficacy of palbociclib in triple-negative breast cancer American journal of cancer research 2022-07-15 [PMID: 35968350] (WB, Human)
• Carstens JL, Yang S, Correa de Sampaio P et al. Stabilized epithelial phenotype of cancer cells in primary tumors leads to increased colonization of liver metastasis in pancreatic cancer Cell reports 2021-04-13 [PMID: 33852841]
• Li J, Pu T, Yin L et al. MAOA-mediated reprogramming of stromal fibroblasts promotes prostate tumorigenesis and cancer stemness Oncogene 2020-02-17 [PMID: 32066880] (IF/IHC, WB, IP)
• Xu G, Yamamoto N, Nojima T, et al. Novel replisome-associated proteins at cellular replication forks in EBV-transformed B lymphocytes PLoS Pathog. 2019-12-01 [PMID: 31841561] (WB)
• Lopez JA Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs Proc. Natl. Acad. Sci. U.S.A. 2019-05-21 [PMID: 31113881] (WB, IHC-P, KD, Mouse)
• Yim SH, Clish CB, Gladyshev VN Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency Cell Rep 2019-05-07 [PMID: 31067459] (ICC/IF, Mouse)

Review for Twist-1 Antibody (NBP2-37364) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 09/27/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Squamous cell carcinoma
• Species: Human
• Lot: 10E4E6

Bioinformatics

• Gene Symbol: TWIST1