ULBP-2/5/6 Antibody (165903) [Unconjugated]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 165903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ULBP-2/5/6 Antibody (165903) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-2
  Accession # Q9BZM5
• Specificity: Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 1 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody (165903) [Unconjugated]

• ALCAN-alpha
• N2DL2
• NKG2DL2
• RAET1H
• RAET1L
• UL16 binding protein 2
• ULBP2
• ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114.
8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-2/5/6 Antibody (MAB1298)(67)

Publications using MAB1298

• Fisher, JG;Doyle, ADP;Graham, LV;Sonar, S;Sale, B;Henderson, I;Del Rio, L;Johnson, PWM;Landesman, Y;Cragg, MS;Forconi, F;Walker, CJ;Khakoo, SI;Blunt, MD; XPO1 inhibition sensitises CLL cells to NK cell mediated cytotoxicity and overcomes HLA-E expression Leukemia 2023-08-01 [PMID: 37528310] (Flow Cytometry, Human)
• Yi Zhang, Feifei Luo, Kuiran Dong Soluble NKG2D ligands impair CD8+ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma Oncology Letters 2023-05-24 [PMID: 37274476]
• Koh, EK;Lee, HR;Son, WC;Park, GY;Kim, J;Bae, JH;Park, YS; Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer Scientific reports 2023-05-11 [PMID: 37169953] (Flow Cytometry, Human)
• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects Nature, 2023-01-11;613(7945):743-750. 2023-01-11 [PMID: 36631610] (Neutralization, Human)
• JD Lewicky, AL Martel, NL Fraleigh, E Picard, L Mousavifar, A Nakamura, F Diaz-Mitom, R Roy, HT Le Exploiting the DNA Damaging Activity of Liposomal Low Dose Cytarabine for Cancer Immunotherapy Pharmaceutics, 2022-12-03;14(12):. 2022-12-03 [PMID: 36559204] (Flow Cytometry, Human)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021-09-04;12(9):836. 2021-09-04 [PMID: 34482362] (Flow Cytometry, Human)
• Daniela A. Covino, Maria G. Desimio, Margherita Doria Combinations of Histone Deacetylase Inhibitors with Distinct Latency Reversing Agents Variably Affect HIV Reactivation and Susceptibility to NK Cell-Mediated Killing of T Cells That Exit Viral Latency International Journal of Molecular Sciences 2021-06-22 [PMID: 34206330]
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021-05-03;17(5):e1008807. 2021-05-03 [PMID: 33939764] (Flow Cytometry, Human)
• E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021-04-06;12(1):2061. 2021-04-06 [PMID: 33824318] (Flow Cytometry, Human)
• Claudia Cantoni, Martina Serra, Erica Parisi, Bruno Azzarone, Angela Rita Sementa, Luigi Aurelio Nasto et al. Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells OncoImmunology 2021-03-24 [PMID: 33758675] (Immunocytochemistry, Human)
• Adeline Raynaud, Klervi Desrumeaux, Laurent Vidard, Elise Termine, Daniel Baty, Patrick Chames et al. Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response OncoImmunology 2021-01-18 [PMID: 33457075]
• Bridget L. Menasche, Eric M. Davis, Shifeng Wang, Yan Ouyang, Suzhao Li, Haijia Yu et al. PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes Science Advances 2020-11-27 [PMID: 33246952]
• Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation Cell Reports 2020-10-13 [PMID: 33053346]
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020-07-20;12(7):. 2020-07-20 [PMID: 32698530] (Flow Cytometry, Human)
• SH Chang, HJ Kim, CG Park Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens Cells, 2020-05-18;9(5):. 2020-05-18 [PMID: 32443511] (Flow Cytometry, Human)
• S Sasawatari, Y Okamoto, A Kumanogoh, T Toyofuku Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells J. Immunol., 2020-01-22;0(0):. 1/22/2020 [PMID: 31969386]
• M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019-08-30;0(0):. 2019-08-30 [PMID: 31471588] (Flow Cytometry, Human)
• Michael T. McCarthy, Da Lin, Tomoyoshi Soga, Julie Adam, Christopher A. O'Callaghan Inosine pranobex enhances human NK cell cytotoxicity by inducing metabolic activation and NKG2D ligand expression European Journal of Immunology 2020-01-01 [PMID: 31434164]
• M Cucè, ME Gallo Cant, MA Siciliano, C Riillo, D Caracciolo, F Scionti, N Staropoli, V Zuccalà, L Maltese, A Di Vito, K Grillone, V Barbieri, M Arbitrio, MT Di Martino, M Rossi, N Amodio, P Tagliaferr, P Tassone, C Botta Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma J Hematol Oncol, 2019-03-21;12(1):32. 2019-03-21 [PMID: 30898137] (Flow Cytometry, Human)
• Erica Giuliani, Maria Giovanna Desimio, Margherita Doria Hexamethylene bisacetamide impairs NK cell-mediated clearance of acute T lymphoblastic leukemia cells and HIV-1-infected T cells that exit viral latency Scientific Reports 2019-03-13 [PMID: 30867508] (Flow Cytometry, Human)
• Maria Giovanna Desimio, Erica Giuliani, Angelo Salvatore Ferraro, Gaspare Adorno, Margherita Doria In Vitro Exposure to Prostratin but Not Bryostatin-1 Improves Natural Killer Cell Functions Including Killing of CD4+ T Cells Harboring Reactivated Human Immunodeficiency Virus Frontiers in Immunology 2018-06-29 [PMID: 30008723]
• Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol 5/29/2018 [PMID: 29910806]
• YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018-05-01;0(0):. 2018-05-01 [PMID: 29518205] (Flow Cytometry, Human)
• S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2017-11-06;7(2):e1392426. 2017-11-06 [PMID: 29308322] (Flow Cytometry, Human)
• MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha Purine nucleotide metabolism regulates expression of the human immune ligand MICA J. Biol. Chem., 2017-12-26;0(0):. 2017-12-26 [PMID: 29279329] (Flow Cytometry, Human)
• Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017-08-17;7(1):8661. 2017-08-17 [PMID: 28819195] (Flow Cytometry, Human)
• C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells Cell Death Dis, 2017-08-03;8(8):e2973. 2017-08-03 [PMID: 28771222] (Flow Cytometry, Human)
• NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model JCI Insight, 2017-07-06;2(13):. 2017-07-06 [PMID: 28679955] (Flow Cytometry, Human)
• X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells Neoplasia, 2017-05-09;19(6):471-482. 2017-05-09 [PMID: 28499126] (Functional Assay, Human)
• S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma Front Immunol, 2017-04-10;8(0):387. 2017-04-10 [PMID: 28443091] (ELISA Development (Detection))
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017-05-09;8(19):31959-31976. 2017-05-09 [PMID: 28404876] (Flow Cytometry, Human)
• Gerald Moncayo, Da Lin, Michael T. McCarthy, Aleksandra A. Watson, Christopher A. O’Callaghan MICA Expression Is Regulated by Cell Adhesion and Contact in a FAK/Src-Dependent Manner Frontiers in Immunology 2017-01-19 [PMID: 28154561]
• Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016-09-27;6(0):32470. 2016-09-27 [PMID: 27671170] (Flow Cytometry, Human)
• Guo M, Wu T, Wan L. Cytotoxic activity of allogeneic natural killer cells on U251 glioma cells in vitro. Mol Med Rep. 5/6/2016 [PMID: 27175912]
  
  Details:
  This citation used the PE version of this antibody.
• Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Onfelt B Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol, 2015-08-28;195(7):3374-81. 2015-08-28 [PMID: 26320254] (Flow Cytometry, Human)
• HYUNKEUN SONG, YEONYE KIM, GABIN PARK, YEONG-SEOK KIM, SEONGHAN KIM, HYUN-KYUNG LEE et al. Transforming growth factor-beta 1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands International Journal of Molecular Medicine 2015-10-01 [PMID: 26311146]
• Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity. Eur J Immunol, 2015-06-09;45(8):2324-34. 2015-06-09 [PMID: 25991034] (Immunoprecipitation, Human)
• Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer, 2015-05-03;15(0):351. 2015-05-03 [PMID: 25933805] (Flow Cytometry, Human)
• Kim , Ga Rim, Ha , Ga-Hee, Bae , Jae-Ho, Oh , Sae-Ock, Kim , Sun-Hee, Kang , Chi-Dug Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells. Oncol Lett, 2015-01-29;9(4):1641-1646. 2015-01-29 [PMID: 25789015] (Flow Cytometry, Human)
• Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. J Immunol, 2015-02-16;194(6):2930-41. 2015-02-16 [PMID: 25687758] (ELISA Development (Detection), Human)
• Baragano Raneros A, Martin-Palanco V, Fernandez A, Rodriguez R, Fraga M, Lopez-Larrea C, Suarez-Alvarez B Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia. Genes Immun, 2014-11-13;16(1):71-82. 2014-11-13 [PMID: 25393931] (Flow Cytometry, Human)
• Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol, 2014-06-09;193(2):950-60. 2014-06-09 [PMID: 24913980] (Flow Cytometry, IHC, Human)
• Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Neutralization, Human)
• Wang , Ruipeng, Sun , Peter D Natural killer cell-mediated shedding of ULBP2. PLoS ONE, 2014-03-10;9(3):e91133. 2014-03-10 [PMID: 24614922] (ICC, Human)
• Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res, 2013-04-11;73(8):2598-607. 2013-04-11 [PMID: 23580577] (Flow Cytometry, Neutralization, Human)
• Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation. Eur J Immunol, 2013-01-15;43(2):550-61. 2013-01-15 [PMID: 23192659] (Flow Cytometry, Human)
• Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Cancer Res, 2012-08-21;72(21):5463-72. 2012-08-21 [PMID: 22915757] (Flow Cytometry, Human)
• Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab. Invest., 2012-03-26;92(7):967-77. 2012-03-26 [PMID: 22449797] (Flow Cytometry, Human)
• Nielsen N, Odum N, Urso B, Lanier LL, Spee P Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 2012-02-22;7(2):e31959. 2012-02-22 [PMID: 22384114] (Flow Cytometry, Human)
• Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012-01-12;8(1):e1002486. 2012-01-12 [PMID: 22253598] (Flow Cytometry, Human)
• Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas Blood 2012-02-09 [PMID: 22167753]
• Delarosa O, Sanchez-Correa B, Morgado S, Ramirez C, del Rio B, Menta R, Lombardo E, Tarazona R, Casado JG Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis. Stem Cells Dev., 2011-10-18;21(8):1333-43. 2011-10-18 [PMID: 21867426] (Flow Cytometry, Human)
• Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene, 2011-01-24;30(23):2622-32. 2011-01-24 [PMID: 21258414] (Flow Cytometry, Human)
• Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells. J. Cell. Sci., 2011-01-11;124(0):321-7. 2011-01-11 [PMID: 21224393] (Flow Cytometry, Human)
• Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia. Leukemia, 2010-04-29;24(6):1152-9. 2010-04-29 [PMID: 20428196] (ELISA Development, Human)
• Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection. Blood, 2010-04-14;115(25):5170-9. 2010-04-14 [PMID: 20393128] (Flow Cytometry, Human)
• Ohashi M, Eagle RA, Trowsdale J Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. J. Biol. Chem., 2010-03-19;285(22):16408-15. 2010-03-19 [PMID: 20304922] (Flow Cytometry, Human)
• Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, Queirolo P, Vermi W, Facchetti F, Moretta A, Moretta L, Mingari MC, Vitale M Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity. Proc. Natl. Acad. Sci. U.S.A., 2009-11-23;0(0):. 2009-11-23 [PMID: 19934056] (Flow Cytometry, Human)
• Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation. Proc. Natl. Acad. Sci. U.S.A., 2009-08-26;106(37):15825-30. 2009-08-26 [PMID: 19717467] (ELISA Development, Human)
• Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC. Int. J. Cancer, 2009-07-01;125(1):212-21. 2009-07-01 [PMID: 19358282] (Flow Cytometry, Human)
• Poggi A, Catellani S, Garuti A, Pierri I, Gobbi M, Zocchi MR Effective in vivo induction of NKG2D ligands in acute myeloid leukaemias by all-trans-retinoic acid or sodium valproate. Leukemia, 2009-01-08;23(4):641-8. 2009-01-08 [PMID: 19151770] (ELISA Development, Human)
• Cerboni C, Ardolino M, Santoni A, Zingoni A Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 2009-01-05;113(13):2955-64. 2009-01-05 [PMID: 19124832] (Neutralization, Human)
• Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int. J. Cancer, 2009-04-01;124(7):1594-604. 2009-04-01 [PMID: 19089914] (Flow Cytometry, Human)
• Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol. Immunol., 2008-07-26;45(15):3917-25. 2008-07-26 [PMID: 18657862] (Flow Cytometry, Human)
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Bioinformatics

• Uniprot:
  • Q9BZM5()