Ulex Europaeus Lectin 1 Native Protein

Product Image: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700] - Lectin from Ulex europaeus (gorse, furze) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.

SDS-Page: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700] - Lectin from Ulex europaeus (gorse, furze) was assessed on SDS-PAGE.

• Reactivity: Pl
• Applications: PAGE, Bioactivity, NULL
• Concentration: LYOPH

Ulex Europaeus Lectin 1 Native Protein Summary

• Description: A biologically active protein corresponding to Ulex Europaeus Lectin 1.
• Details of Functionality: Agglutination activity is expressed in ug/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 degrees C.
• Protein/Peptide Type: Native Protein
• Purity: >50% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Product Image
  • SDS-Page
• Application Notes: Ulex europaeus agglutinin has anti-H blood group specificity.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Lyophilized in buffer. Information about buffer components is properietary.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >50% SDS-PAGE
• Reconstitution Instructions: Reconstitute with PBS (pH 6.8) to a final concentration of 1mg/ml.

Alternate Names for Ulex Europaeus Lectin 1 Native Protein

• Gorse or Furze Lectin
• UEA-1; H(O) lectin 1; Anti H(O) lectin I

Background

Ulex europaeus agglutinin I, a lectin, binds to many glycoproteins and glycolipids containing alpha-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells.There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N'-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.