ULK1 Antibody

Western Blot: ULK1 Antibody [NBP2-24738] - Analysis of ULK1 in Raji cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody.

Immunohistochemistry-Paraffin: ULK1 Antibody [NBP2-24738] - Analysis of human kidney using NBP2-24738

Western Blot: ULK1 Antibody [NBP2-24738] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.

Product Discontinued

ULK1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids between 320 and 370 of human ULK1 was used as the immunogen, GenBank no. sp O75385.2 ULK1_HUMAN.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ULK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 27740626)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ULK1 Antibody

• ATG1 autophagy related 1 homolog
• ATG1
• ATG1A
• EC 2.7.11
• EC 2.7.11.1
• FLJ38455
• FLJ46475
• hATG1
• KIAA0722
• serine/threonine-protein kinase ULK1
• ULK1
• unc-51 (C. elegans)-like kinase 1
• UNC51
• Unc51.1
• unc-51-like kinase 1 (C. elegans)
• Unc-51-like kinase 1

Background

ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULK1 Antibody (NBP2-24738)(10)

Publications using NBP2-24738

• Zhang F, Wang J, Chu J et al. MicroRNA-146a Induced by Hypoxia Promotes Chondrocyte Autophagy through Bcl-2. Cell. Physiol. Biochem. 2015-11-06 [PMID: 26492575] (WB, Mouse)
• Karanasios E, Ktistakis NT. Studying Autophagy: List of Useful Antibodies Produced via a Community Effort. Autophagy at the Cell, Tissue and Organismal Level. 2016-04-24
• Idera, A;Sharkey, LM;Kurauchi, Y;Kadoyama, K;Paulson, HL;Katsuki, H;Seki, T; Wild-type and pathogenic forms of ubiquilin 2 differentially modulate components of the autophagy-lysosome pathways Journal of pharmacological sciences 2023-07-01 [PMID: 37257946] (Western Blot, Human)
• Da Silva Tf, Granadeiro Ls, Bessa-Neto D Et Al. Plasmalogens regulate the AKT-ULK1 signaling pathway to control the position of the axon initial segment Progress in neurobiology 2021-07-21 [PMID: 34302896] (WB)
• Sacitharan P, Bou-Gharios G, Edwards J SIRT1 directly activates autophagy in human chondrocytes Cell Death Discov. 2020-12-01 [PMID: 32528730] (WB, Human)
• Franco-Iborra S, Plaza-Zabala A, Montpeyo M et al. Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease Autophagy 2020-02-24 [PMID: 32093570]
• Chen G, Gao X, Wang J et al. Hypoxia-induced microRNA-146a represses Bcl-2 through Traf6/IRAK1 but not Smad4 to promote chondrocyte autophagy. Biol. Chem. 2016-12-19 [PMID: 27845876]
• Ovadje P, Ammar S, Guerrero Ja et al. Dandelion root extract affects colorectal cancer proliferation and survival through the activation of multiple death signalling pathways. Oncotarget 2016-08-22 [PMID: 27564258] (WB)
• Martinez-Vicente M, Franco-Iborra S, Plaza-Zabala A et al. Impairment of neuronal mitophagy by mutant huntingtin bioRxiv 2018-05-24
• Chen Zhen-Hua, Wang Wen-Tao, Huang Wei et al. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway. Cell Death and Differentiation 2016-10-14 [PMID: 27740626] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: ULK1
• Uniprot:
  • O75385(Human)