Recombinant Human USAG1/SOSTDC1 His Protein

SDS-Page: SOSTDC1 Protein [NBP2-23453]

Product Discontinued

Recombinant Human USAG1/SOSTDC1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-206 of Human USAG1/SOSTDC1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFKNDATE ILYSHVVKPV PAHPSSNSTL NQARNGGRHF SNTGLDRNTR VQVGCRELRS TKYISDGQCT SISPLKELVC AGECLPLPVL PNWIGGGYGT KYWSRRSSQE WRCVNDKTRT QRIQLQCQDG STRTYKITVV TACKCKRYTR QHNESSHNFE SMSPAKPVQH HRERKRASKS SKHSMS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SOSTDC1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. Use in WB reported in scientific literature (PMID 24667598)
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human USAG1/SOSTDC1 His Protein

• CDA019
• cystine-knot containing secreted protein
• DKFZp564D206
• Ectodermal BMP inhibitor
• Ectodin
• sclerostin domain containing 1
• sclerostin domain-containing protein 1
• SOSTDC1
• USAG1
• USAG-1
• USAG1uterine sensitization-associated protein-1
• Uterine sensitization-associated gene 1 protein
• WISE

Background

SOSTDC1 is a member of the sclerostin family and an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death. Recombinant human SOSTDC1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for USAG1/SOSTDC1 Protein (NBP2-23453)(1)

Publication using NBP2-23453

• Hernandez P, Whitty C, John Wardale R, Henson FM. New insights into the location and form of sclerostin. Biochem. Biophys. Res. Commun. 2014-03-22 [PMID: 24667598] (WB)

Bioinformatics

• Gene Symbol: SOSTDC1