Varicella Zoster Virus Antibody (SG4)

Product Discontinued

Varicella Zoster Virus Antibody (SG4) Summary

• Immunogen: VZV Ellen Strain from VZV-infected monkey kidney cells (BSC-1).
• Localization: Cell Membrane and Cytoplasmic
• Specificity: Reacts with VZV glycoprotein IV (VZVgI) and to a lesser extent VZV glycoprotein I (VZVgE) by immunoprecipitation test. This clone reacts with both precursor and mature glycoprotein IV (VZVgI).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation

Reactivity Notes

Cross-reacts with Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Sodium Phosphate (pH 9.0)
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Varicella Zoster Virus Antibody (SG4)

• Envelope glycoprotein gI
• Gi
• Glycoprotein IV
• GPIV
• HHV 3
• HHV3
• HHV3gp39
• Human herpes virus 3
• Human herpesvirus 3
• Membrane glycoprotein gE
• VZV
• VZVgE
• VZVgI

Background

Varicella Zoster Virus (VZV), a member of the human herpes virus family, causes two distinct clinical manifestations: childhood chickenpox(Varicella) and shingles (zoster). Varicella is the outcome of the primary infection with VZV, whereas, zoster is the result of VZV reactivation from latently infected sensory ganglia which occurs predominantly in aging and immunosuppressed individuals. VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV, however there is no equivalent of HSV gD. VZV virons are spherical and 150-200 nm in diameter. Its lipid envelope encloses the nucleocapsid of 162 capsomeres arranged in a hexagonal form. Its DNA is a single linear, double strand molecule, 125,000 nt long. Glycoprotein E (gE), a 623 amino acid type I integral membrane protein, is expressed on plasma membranes and in the cytoplasm of VZV infected cells, where it is presumed to be present in the membranes of intracellular vesicles or organelles. VZV gE complexes with gI in the rough endoplasmic reticulum, and most gE exists in the form of gE to gI heterodimers in infected cells. VZV gE is also a predominant component of the virion envelope. The functions of gE, which is encoded by ORF68, are of particular interest because it has been demonstrated that ORF68 is essential for VZV replication. In contrast, the genes for gE proteins can be deleted from herpes simplex virus and pseudorabies virus, albeit with significant reductions in infectivity in cell culture and in animal models. Since the VZV genome does not encode a homologue of gD, VZV gE may have functions that are usually segregated between gD and gE, or the gE to gI complex, in other alphaherpesviruses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.