VCAM-1/CD106 Antibody (1.4C3)

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Detection of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.

ELISA: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Analysis (in-cell) of HDMVE cells using HRP conjugated VCAM-1 antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 1.4C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

VCAM-1/CD106 Antibody (1.4C3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33182)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone 1.4C3 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human umbilical vein endothelial cells (HUVEC)
• Localization: Cell surface
• Marker: Activated Endothelial Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for VCAM-1/CD106 Antibody (1.4C3)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

VCAM1 (vascular cell adhesion molecule-1, also known as CD106 and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM1 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP2-29413)(2)

Publications using NBP2-29413

• Troupin A, Shirley D, Londono-Renteria B et al. A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread. J Immunol. 2016-12-01 [PMID: 27799312] (ELISA, Human)
  
  Details:
  This citation used the HRP version of this antibody.
• Yurdakan G, Ekem TE, Bahadir B et al. Expression of adhesion molecules in first trimester spontaneous abortions and their role in abortion pathogenesis. Acta Obstet Gynecol Scand. 2008-01-01 [PMID: 18607815]

Bioinformatics

• Gene Symbol: VCAM1
• Uniprot:
  • P19320()
  • P19320()
  • P19320()