VCAM-1/CD106 Antibody (6G9) - BSA Free

Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Photomicrographs of two separate gut sections from a patient with EHEC colitis. Panels (C) and (D) are stained to show VCAM-1/CD106 expression in endothelium, indicating inflammatory activation (40x magnification). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0055278), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.

Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Photomicrographs of two separate gut sections from a patient with EHEC colitis.Panels (A) and (B) are stained with CD31 to enumerate endothelium lining the vessels (40× magnification). (C) and (D) are stained to show VCAM-1 expression in endothelium, indicating inflammatory activation (40× magnification).

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Enhanced expression of endothelial SENP1 & GATA2 correlates w/ graft arteriosclerosis (GA) progression.Similarly sized human coronary arteries w/ GA from chronically rejecting heart allografts or w/out disease from non-transplanted hearts collected & evaluated by histological analysis. (a,b) Dramatically increased expression of endothelial SENP1 detected in the diseased vessel wall. Endothelial SENP1 expression is demonstrated by immunofluorescence analysis of coronary artery cross-sections that stained for SENP1 & the endothelial marker PECAM-1 w/ DAPI labelling of the nuclei. Representative images are shown in (a) w/ quantification data in (b). Bar represents 50 μm. (c) Induction of endothelial adhesion molecules resulted in a similar augmented pattern as endothelial SENP1. Representative images of immunofluorescence staining for VCAM-1 or P-selectin & PECAM-1 in coronary arteries w/ DAPI counterstaining are shown. Bar represents 50 μm. (d,e) Expression of endothelial GATA2 elevated w/ the progression of aggravated rejection. Representative images of the immunofluorescence staining of coronary arteries for GATA2 & PECAM-1 together w/ DAPI nuclear staining are shown in (d) w/ quantification data in (e). Negative SENP1 & GATA2 staining in the isotype or blocking peptide controls are also shown in (a) & (d). Bar represents 50 μm. Data presented in (b,e) are the mean±s.e.m. from five separate clinical specimens per group as indicated. *P<0.05, **P<0.01 & ***P<0.0001; one-way ANOVA followed by Bonferroni test. GA, graft arteriosclerosis; PECAM, platelet/endothelial cell adhesion molecule; SENP, sentrin-specific protease; VCAM, vascular cell adhesion molecule. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Loss of endothelial SENP1 inhibits EC activation.(a) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin & PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 μm. (b–e) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 & P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1 beta treatment. Representative histograms are shown in (b) with the quantification of mean intensity in (c–e). (f–h) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines & analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 & VCAM-1 are shown in (f) with the quantification of mean intensity in (g,h). Data are presented as the mean±s.e.m. from at least three independent experiments. *P<0.05 & **P<0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ELISA, IHC, IP, WB
• Clone: 6G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

VCAM-1/CD106 Antibody (6G9) - BSA Free Summary

• Immunogen: This VCAM-1/CD106 Antibody (6G9) was developed against a purified recombinant fragment of human VCAM-1 expressed in E. coli. [Uniprot: P19320]
• Localization: Membrane. Single-pass type I membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunoblotting reported in scientific literature (PMID 28569748)
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation reported in scientific literature (PMID 28569748)
  • Simple Western 1:1000
  • Western Blot 1:500-1:2000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HUVEC lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID: 32243809).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for VCAM-1/CD106 Antibody (6G9) - BSA Free

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP1-47491)(5)

Publications using NBP1-47491

• Park, JY;Mani, S;Clair, G;Olson, HM;Paurus, VL;Ansong, CK;Blundell, C;Young, R;Kanter, J;Gordon, S;Yi, AY;Mainigi, M;Huh, DD; A microphysiological model of human trophoblast invasion during implantation Nature communications [PMID: 35292627] (ICC/IF, Human)
• Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse)
• Qiu C, Wang Y, Zhao H et al. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nat Commun. 2017-06-01 [PMID: 28569748] (IP, IB, Human)
• Chen PY, Qin L, Baeyens N et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest 2015-10-26 [PMID: 26517696] (WB)
• Ullrich S, Bremer P, Neumann-Grutzeck C et al. Symptoms and Clinical Course of EHEC O104 Infection in Hospitalized Patients: A Prospective Single Center Study. PLoS One 2013-01-01 [PMID: 23460784] (IHC-P, Human)

Reviews for VCAM-1/CD106 Antibody (NBP1-47491) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 10/13/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: FFPE,human lung cancer
• Species: Human
• Lot: A-4

reviewed by: Bryan Tinsley IHC Human 04/21/2014

Summary

• Application: Immunohistochemistry
• Species: Human

Bioinformatics

• Gene Symbol: VCAM1
• Entrez:
  • 7412(Human)
• Uniprot:
  • P19320()