VECTASTAIN® ELITE® ABC anti-mouse/rabbit, R.T.U. (Ready to Use) HRP Immunodetection Kit

• Reactivity: Mu, Rb
• Applications: WB, ELISA, Func, ICC/IF, IHC, ELISA

VECTASTAIN® ELITE® ABC anti-mouse/rabbit, R.T.U. (Ready to Use) HRP Immunodetection Kit Summary

• Description: Features:
• Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
• Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
• Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
• Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
• Kit Type: HRP Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • ELISA
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
  • Western Blot
• Application Notes: Contains sufficeint reagent to stain approximately 250-500 sections.
  Note:ur Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.