Vector Transfected HEK 293 Stable Cell Line

Product Discontinued

Vector Transfected HEK 293 Stable Cell Line Summary

• Description: The Vector/HEK 293 cell line can be used as the negative vector control for the TLR/HEK 293 cell lines (NBP2-26264, NBP2-26266, NBP2-26267, NBP2-26268, NBP2-26269, NBP2-26293, NBP2-26270, NBP2-26271).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: Vector/HEK 293 is a stably transfected cell line with a blank vector.
• Specificity: Vector Control Stably Transfected HEK 293 Cells (negative control for transfected TLR cell lines)
• Selection Agent: Blasticidin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Stable Cell Line
• Host: HEK293

Applications/Dilutions

Publications

Read Publications using NBP2-26259.

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Related Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the vector control stable line.
- Wash hands after handling the vector control stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Publications for Vector Transfected HEK 293 Reporter Cell Line (NBP2-26259)(2)

Publications using NBP2-26259

• Mauldin IS, Wang E, Deacon DH et al. TLR2/6 agonists and interferon-gamma induce human melanoma cells to produce CXCL10 Int. J. Cancer 2015-03-12 [PMID: 25765738]
• Feng D, Sangster-Guity N, Stone R et al. Differential requirement of histone acetylase and deacetylase activities for IRF5-mediated proinflammatory cytokine expression. J Immunol. 2010-11-15 [PMID: 20935208]