Recombinant Human VEGF 165 Protein

Product Discontinued

Recombinant Human VEGF 165 Protein Summary

• Description: A disulfide-linked homodimeric protein consisting of two 166 amino acid polypeptide chains with Met at N-terminus corresponding to VEGF 165 Source: E. coli

  Uniprot ID: P15692

  Amino Acid Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

• Details of Functionality: VEGF 165 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 0.1 EU/ug of VEGF 165 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 38.6 (homodimer) kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, with 0.02% Tween-20, pH 7.0.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.33 mg/mL.

Alternate Names for Recombinant Human VEGF 165 Protein

• MVCD1
• vascular endothelial growth factor A
• Vascular permeability factor
• VEGF-A
• VEGFMGC70609
• VPFvascular endothelial growth factor

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (a.a.) in length. VEGF production can be induced in cells that are not receiving enough oxygen. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Recombinant human VEGF165 contains 166 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 88 % a.a. with corresponding regions of mouse and rat, 96 % with porcine, 95 % with canine, and 93 % with feline, equine and bovine VEGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for VEGF 165 Recombinant Protein (NBP2-35135)(2)

Publications using NBP2-35135

• Yanlan Yu, Pierre Lee, Yaohuang Ke et al. Development of humanized rabbit monoclonal antibodies against vascular endothelial growth factor receptor 2 with potential antitumor effects. Biochem Biophys Res Commun 2013-07-05 [PMID: 23770369]
• Yanlan Yu, Pierre Lee, Yaohuang Ke et al. A Humanized Anti-VEGF Rabbit Monoclonal Antibody Inhibits Angiogenesis and Blocks Tumor Growth in Xenograft Models. PLoS One 2010-02-05 [PMID: 20140208]

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • P15692()