VEGF-C Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for VEGF-C at approximately 52 kDa, 34 kDa, and 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VEGF-C was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to stromal cells surrounding crypts in the colon mucosa (cross section across crypts). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGF-C was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in crypts of the colon mucusa (longitudinal section of crypts). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human VEGF‑C protein was serially diluted 2-fold and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (MAB752) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VEGF-C Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VEGF-C
• Specificity: Detects human VEGF-C in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VEGFC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF-C Antibody [Unconjugated]

• Flt4 ligand
• Flt4-L
• vascular endothelial growth factor C
• Vascular endothelial growth factor-related protein
• VEGFC
• VEGF-C
• VRPFLT4 ligand DHM

Background

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF sub-family that share the conserved VEGF homology domain (VHD) with other VEGF family members but are distinguished by their preferential formation of non-covalent homodimers. Both VEGF-C and -D have long N- and C-terminal propeptide extensions. The VEGF-C propeptide undergoes stepwise proteolytic processing to generate ligands with increasing affinity for VEGF-R3. However, only the fully processed VEGF-C containing just the VHD can bind VEGF-R2. None of the VEGF-C forms have appreciable affinity for VEGF-R1. VEGF-C is expressed in multiple adult human tissues, most prominently in lymph nodes, heart, placenta, ovary, and small intestine. Traces of VEGF-C are also detected in brain, liver, thymus, skeletal muscles, spleen, prostate, testis and colon. Unlike other VEGF family members, VEGF-C expression is not regulated by hypoxia. VEGF-C is a lymphangiogenic growth factor and the VEGF-C/VEGF-R3 signaling pathway has been shown to be crucial for lymphangiogenesis. VEGF-C and VEGF-R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. VEGF-C stimulates lymphangiogenesis in the avian chorioallantoic membrane model. Over-expression of VEGF-C in breast cancer cells has been shown to increase intratumoral lymphangiogenesis, resulting in enhanced metastasis to regional lymph nodes and to the lungs. Mouse tumors expressing elevated levels of VEGF-C have increased lymphatic metastasis and increased lymphatic surface area in the tumor margin. VEGF-C is also associated with lymph node metastasis of colorectal carcinoma. Besides lymphangiogenesis, VEGF-C can have potent effects on physiological angiogenesis through its interaction with VEGF R2. The protein can stimulate migration and proliferation of endothelial cells in vitro and in vivo and has been shown to stimulate angiogenesis in the mouse cornea and in rabbit hind limb ischaemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGF-C Antibody (AF752)(29)

Publications using AF752

• Qi, L;Li, X;Zhang, F;Zhu, X;Zhao, Q;Yang, D;Hao, S;Li, T;Li, X;Tian, T;Feng, J;Sun, X;Wang, X;Gao, S;Wang, H;Ye, J;Cao, S;He, Y;Wang, H;Wei, B; VEGFR-3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection Cell reports 2023-05-10 [PMID: 37167063] (IHC, Mouse)
• Nadejda Korneeva, Md Imtiaz Khalil, Ishita Ghosh, Ruping Fan, Thomas Arnold, Arrigo De Benedetti SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions Virology Journal 3/30/2023 [PMID: 36998012]
• Aya Izumi, Koji Yamamoto, Makiko Kawaguchi, Fumiki Yamashita, Tsuyoshi Fukushima, Takumi Kiwaki et al. Insufficiency of hepatocyte growth factor activator inhibitor‐1 confers lymphatic invasion of tongue carcinoma cells Cancer Science 6/1/2022 [PMID: 35332604]
• L Dupont, L Joannes, F Morfoisse, S Blacher, C Monseur, CF Deroanne, A Noël, AC Colige ADAMTS2 and ADAMTS14 substitute ADAMTS3 in adults for proVEGFC activation and lymphatic homeostasis JCI Insight, 2022-04-22;0(0):. 2022-04-22 [PMID: 35316211] (Western Blot, Human)
• D Sz?ke, G Kovács, É Kemecsei, L Bálint, K Szoták-Ajt, P Aradi, A Styevkóné, BL Mui, YK Tam, TD Madden, K Karikó, RP Kataru, MJ Hope, D Weissman, BJ Mehrara, N Pardi, Z Jakus Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema Nature Communications, 2021-06-08;12(1):3460. 2021-06-08 [PMID: 34103491] (Western Blot, Human)
• I Hwang, JW Kim, K Ylaya, EJ Chung, H Kitano, C Perry, J Hanaoka, J Fukuoka, JY Chung, SM Hewitt Tumor-associated macrophage, angiogenesis and lymphangiogenesis markers predict prognosis of non-small cell lung cancer patients J Transl Med, 2020-11-23;18(1):443. 2020-11-23 [PMID: 33228719] (IHC, Human)
• G Wang, L Muhl, Y Padberg, L Dupont, J Peterson-M, M Stehling, F le Noble, A Colige, C Betsholtz, S Schulte-Me, A van Impel Specific fibroblast subpopulations and neuronal structures provide local sources of Vegfc-processing components during zebrafish lymphangiogenesis Nat Commun, 2020-06-01;11(1):2724. 2020-06-01 [PMID: 32483144] (Western Blot, Human)
• E Song, T Mao, H Dong, LSB Boisserand, S Antila, M Bosenberg, K Alitalo, JL Thomas, A Iwasaki VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours Nature, 2020-01-15;577(7792):689-694. 2020-01-15 [PMID: 31942068] (Human)
• M Rudzi?ska, M Mikula, KD Arczewska, E Gajda, S Sabali?ska, T St?pie?, J Ostrowski, B Czarnocka Transcription Factor Prospero Homeobox 1 (PROX1) as a Potential Angiogenic Regulator of Follicular Thyroid Cancer Dissemination Int J Mol Sci, 2019-11-10;20(22):. 2019-11-10 [PMID: 31717665] (Western Blot, Human)
• SK Jha, K Rauniyar, E Chronowska, K Mattonet, EW Maina, H Koistinen, UH Stenman, K Alitalo, M Jeltsch KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D Elife, 2019-05-17;8(0):. 5/17/2019 [PMID: 31099754]
• Han Jo Kim, Moo-Jun Baek, Dong Hyun Kang, Sang-Cheol Lee, Sang Byung Bae, Kyu Taek Lee et al. Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer Annals of Coloproctology 4/30/2018 [PMID: 29742856]
• P Åström, K Juurikka, ES Hadler-Ols, G Svineng, NK Cervigne, RD Coletta, J Risteli, JH Kauppila, S Skarp, S Kuttner, A Oteiza, M Sutinen, T Salo The interplay of matrix metalloproteinase-8, transforming growth factor-?1 and vascular endothelial growth factor-C cooperatively contributes to the aggressiveness of oral tongue squamous cell carcinoma Br. J. Cancer, 2017-08-03;117(7):1007-1016. 2017-08-03 [PMID: 28772283] (IHC, Western Blot, Human)
• SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1 Sci Rep, 2017-07-07;7(1):4916. 2017-07-07 [PMID: 28687807] (Western Blot, Primate - Chlorocebus aethiops (African Green Monkey))
• H Kitano, JY Chung, KH Noh, YH Lee, TW Kim, SH Lee, SH Eo, HJ Cho, CH Choi, S Inoue, J Hanaoka, J Fukuoka, SM Hewitt Synaptonemal complex protein 3 is associated with lymphangiogenesis in non-small cell lung cancer patients with lymph node metastasis J Transl Med, 2017-06-17;15(1):138. 2017-06-17 [PMID: 28623914] (IHC, Western Blot, Human)
• Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors Science Advances 10/28/2016 [PMID: 27819042]
• Yoko Dong, Satoru Kase, Zhenyu Dong, Junichi Fukuhara, Yoshiaki Tagawa, Erdal Tan Ishizuka et al. Regulation of vascular endothelial growth factor-C by tumor necrosis factor-alpha in the conjunctiva and pterygium International Journal of Molecular Medicine 8/1/2016 [PMID: 27314284]
• Lan Zhang, Jian-Hua Wang, Rong-Xin Liang, Shu-Ting Huang, Jing Xu, Lin-Jing Yuan et al. RASSF8 downregulation promotes lymphangiogenesis and metastasis in esophageal squamous cell carcinoma Oncotarget 9/30/2015 [PMID: 26439687]
• Lan Zhang, Jian-Hua Wang, Rong-Xin Liang, Shu-Ting Huang, Jing Xu, Lin-Jing Yuan et al. RASSF8 downregulation promotes lymphangiogenesis and metastasis in esophageal squamous cell carcinoma Oncotarget 2015-09-30 [PMID: 26439687] (Immunohistochemistry, Western Blot, Human)
• Carpenter RL, Paw I, Zhu H et al. The gain-of-function GLI1 transcription factor TGLI1 enhances expression of VEGF-C and TEM7 to promote glioblastoma angiogenesis. Oncotarget 9/8/2015 [PMID: 26093087]
• Lewin J, Khamly K, Young R, Mitchell C, Hicks R, Toner G, Ngan S, Chander S, Powell G, Herschtal A, Te Marvelde L, Desai J, Choong P, Stacker S, Achen M, Ferris N, Fox S, Slavin J, Thomas D A phase Ib/II translational study of sunitinib with neoadjuvant radiotherapy in soft-tissue sarcoma. Br J Cancer, 2014-10-16;111(12):2254-61. 2014-10-16 [PMID: 25321190] (IHC, Human)
• JINGHUI GUO, WENHUI LOU, YUAN JI, SHUNCAI ZHANG Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma Oncology Letters 5/1/2013 [PMID: 23761820]
• Marissa J. Stanton, Samikshan Dutta, Heyu Zhang, Navatha Shree Polavaram, Alexey A. Leontovich, Pia Hönscheid et al. Autophagy Control by the VEGF-C/NRP-2 Axis in Cancer and Its Implication for Treatment Resistance Cancer Research 1/1/2013 [PMID: 23149913]
• Lin CE, Chen SU, Lin CC Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells. PLoS ONE, 2012-07-20;7(7):e41096. 2012-07-20 [PMID: 22911748] (Western Blot, Human)
• Junichi Fukuhara, Satoru Kase, Tsutomu Ohashi, Ryo Ando, Zhenyu Dong, Kousuke Noda et al. Expression of vascular endothelial growth factor C in human pterygium Histochemistry and Cell Biology 2/1/2013 [PMID: 22910845]
• Donoghue JF, McGavigan CJ, Lederman FL Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding. PLoS ONE, 2012-02-17;7(2):e30916. 2012-02-17 [PMID: 22383980] (Western Blot, Human)
• Matsuura M, Onimaru M, Yonemitsu Y, Suzuki H, Nakano T, Ishibashi H, Shirasuna K, Sueishi K Autocrine loop between vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 positively regulates tumor-associated lymphangiogenesis in oral squamoid cancer cells. Am. J. Pathol., 2009-09-24;175(4):1709-21. 2009-09-24 [PMID: 19779139] (ICC, Human)
• Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergun S Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1. Blood, 2007-08-30;110(13):4223-33. 2007-08-30 [PMID: 17761831] (Western Blot, Human)
• Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nat. Neurosci., 2006-02-05;9(3):340-8. 2006-02-05 [PMID: 16462734] (IHC-Fr, Mouse)
• Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma. Cancer Res., 2004-01-15;64(2):554-64. 2004-01-15 [PMID: 14744769] (IHC-P, Western Blot, Human)

Review for VEGF-C Antibody (AF752) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23423575
• Species: Human

FAQs for VEGF-C Antibody (AF752). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: VEGFC