![Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273] - Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VHH. Primary antibody: Rabbit anti-Llama VHH Secondary Antibody at 1:1,000 at 4C. Secondary antibody: HRP Goat anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.](http://images.novusbio.com/fullsize/Rabbit-anti-Llama-VHH-Antibody-Secondary-Antibody-Western-Blot-NBP2-95273-img0001.jpg "Western Blot: Rabbit anti-Llama VHH Secondary Antibody [NBP2-95273] - Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VHH. Primary antibody: Rabbit anti-Llama VHH Secondary Antibody at 1:1,000 at 4C. Secondary antibody: HRP Goat anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.")
| Reactivity | LlSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Rabbit anti-Llama VHH Secondary Antibodywas prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein. |
| Specificity | Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-Vhh antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
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Good Things Come in Small Packages: The Power of LlaMABody-Camelid Antibodies By Jennifer Jones, M.S.When you think of antibody producing animals in the lab you probably think of rabbits, mice, goats, and rats. But what about llamas? Llamas probably aren’t the first animal, or even th... Read full blog post. |
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