Vimentin Antibody (280618) [Unconjugated]

Vimentin was detected in immersion fixed paraffin-embedded sections of human colon using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 1 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

Vimentin was detected in immersion fixed paraffin-embedded sections of human colon using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 1 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.

Vimentin was detected in immersion fixed paraffin-embedded sections of human liver using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 1 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.​

Vimentin was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 1 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder.​​

Vimentin Antibody was detected in immersion fixed paraffin-embedded sections of Mouse Kidney using Rat Anti-Mouse Vimentin, Monoclonal Antibody (Catalog # MAB2105) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of MEF mouse embryonic feeder cells, NIH-3T3 mouse embryonic fibroblast cell line, Rat-2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vimentin was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Vimentin was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Vimentin was detected in immersion fixed mouse cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Vimentin was detected in immersion fixed rat cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Vimentin was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 58 kDa (as indicated) using 10 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

A very minimal population of human vimentin+ HSCs/myofibroblasts express a primary cilium, with none detected on CD31+ endothelial cells.Human ALD liver tissue was examined for the expression of primary cilia ( alpha -acetylated tubulin, green; gamma -tubulin, red) by vimentin+ (grey) HSCs/myofibroblasts (A) or CD31+ (grey) ECs (C). (A) The majority of vimentin+ cells were Pc-ve in the tissues examined. Representative image shown, displaying absence of Pc on vimentin+ cells. To confirm this result, ciliary protein Arl13b (green) was co-stained with vimentin (grey). Rare Arl13b ciliary structures (arrow) co-localised with vimentin+ cells. Final panel in A illustrates rare Pc+ ( alpha -acetylated tubulin, green; gamma -tubulin, red) vimentin+ (grey) HSCs/myofibroblasts, at the cirrhotic interface. (B) Number of vimentin+ Pc+ cells or vimentin+ Pcneg cells per FOV (n = 3 ALD samples, 8 FOV/sample). (C) No Pc were detected on CD31+ cells in the tissues examined (ALD n = 3, 8 FOV/sample). Representative image shown. All images obtained using confocal microscopy, 63x objective. DAPI, blue. White arrows illustrate Pc. * Non-specific liver autofluorescence. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Widespread GLI expression in human donor and cirrhotic liver.(A) Frozen (4 μm) human donor (n = 5), and cirrhotic liver sections [ALD (n = 6), NASH (n = 3), PBC (n = 1)] were screened for GLI2 (red) expression by immunofluorescence. Representative images taken at 5x or 40x (insets) objective shown. DAPI, blue. (B) qRT-PCR for GLI1 and GLI3 transcript in human donor or ALD samples. Mean±S.E.M. Significant (*) difference between means (One-sided student t-test, **p150 kDa) in donor (Don) or ALD patient samples. Densitometry analysis with GLI1 normalised to GAPDH (Image J). Mean±S.E.M; **p = 0.0093 (Two-sided student t-test). (C) Nuclear GLI2 (green) expression in EpCAM+ (red) LPCs in donor, ALD, PBC and NASH liver. (D) Nuclear GLI2 (green) expression demonstrated within CD31+ (red) ECs, CK18+ (red) hepatocytes, CD45+ (red) leukocytes and vimentin+ (red) HSCs/myofibroblasts, in ALD. 63x objective. (E) Maximum intensity projection illustrating close physical association between EpCAM+ LPCs (green) and vimentin+ HSCs/myofibroblasts (red), both of which express GLI2 (grey), in ALD tissue. Arrows indicate myofibroblasts directly contacting LPCs. Confocal microscopy, 63x objective. Quantitation (%) of EpCAM+ GLI2+ cells and vimentin+ GLI2+ cells within the same FOV (n = 3 ALD samples, 8 FOV/sample). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Vimentin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, Flow, ICC/IF, KO, mIF
• Clone: 280618
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Vimentin Antibody (280618) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Vimentin
  Ser2-Glu466
  Accession # P08670
• Specificity: Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: VIM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Multiplex Immunofluorescence 1-10 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1-2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vimentin Antibody (280618) [Unconjugated]

• epididymis secretory sperm binding protein
• FLJ36605
• VIM
• Vimentin

Background

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
8. Styers, M.L. et al. (2005) Traffic 6:359.
9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
15. Garg, A. et al. (2006) J. Immunol. 177:6192.

Show all background references

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vimentin Antibody (MAB2105)(45)

Publications using MAB2105

• Prondzynski, M;Berkson, P;Trembley, MA;Tharani, Y;Shani, K;Bortolin, RH;Sweat, ME;Mayourian, J;Yucel, D;Cordoves, AM;Gabbin, B;Hou, C;Anyanwu, NJ;Nawar, F;Cotton, J;Milosh, J;Walker, D;Zhang, Y;Lu, F;Liu, X;Parker, KK;Bezzerides, VJ;Pu, WT; Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems Nature communications 2024-07-15 [PMID: 39009604] (Immunocytochemistry, Human)
• Song, X;Nihashi, Y;Imai, Y;Mori, N;Kagaya, N;Suenaga, H;Shin?ya, K;Yamamoto, M;Setoyama, D;Kunisaki, Y;Kida, Y; Collagen Lattice Model, Populated with Heterogeneous Cancer-Associated Fibroblasts, Facilitates Advanced Reconstruction of Pancreatic Cancer Microenvironment International Journal of Molecular Sciences 2024-03-27 [PMID: 38612551] (Immunohistochemistry, Human)
• Abubaker, M;Greaney, A;Newport, D;Mulvihill, JJE; Characterization of primary human leptomeningeal cells in 2D culture Heliyon 2024-03-15 [PMID: 38434413] (Immunocytochemistry, Human)
• Ana López-Guajardo, Azeer Zafar, Khairat Al Hennawi, Valentina Rossi, Abdulaziz Alrwaili, Jessica D. Medcalf et al. Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6 Frontiers in Molecular Biosciences 2023-07-20 [PMID: 37564130]
• Shimizu, K;Kikuta, J;Ohta, Y;Uchida, Y;Miyamoto, Y;Morimoto, A;Yari, S;Sato, T;Kamakura, T;Oshima, K;Imai, R;Liu, YC;Okuzaki, D;Hara, T;Motooka, D;Emoto, N;Inohara, H;Ishii, M; Single-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction Nature communications 2023-08-03 [PMID: 37537159] (Immunohistochemistry, Human)
• Park, GT;Lim, JK;Choi, EB;Lim, MJ;Yun, BY;Kim, DK;Yoon, JW;Hong, YG;Chang, JH;Bae, SH;Ahn, JY;Kim, JH; Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia Biomaterials research 2023-07-16 [PMID: 37455318] (Flow Cytometry, Human)
• Shiju TM, Sampaio LP, Hilgert GSL, Wilson SE Corneal epithelial basement membrane assembly is mediated by epithelial cells in coordination with corneal fibroblasts during wound healing Molecular vision 2023-05-20 [PMID: 37287640]
• Singh, DK;Carcamo, S;Farias, EF;Hasson, D;Zheng, W;Sun, D;Huang, X;Cheung, J;Nobre, AR;Kale, N;Sosa, MS;Bernstein, E;Aguirre-Ghiso, JA; 5-Azacytidine- and retinoic-acid-induced reprogramming of DCCs into dormancy suppresses metastasis via restored TGF-?-SMAD4 signaling Cell reports 2023-05-29 [PMID: 37267946] (IHC, Mouse)
• Polisetti, N;Reinhard, T;Schlunck, G; Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering International journal of molecular sciences 2023-04-25 [PMID: 37175529] (ICC, Human)
• Hyejeong Rosemary Kim, Samantha J. Warrington, Ana López-Guajardo, Khairat Al Hennawi, Sarah L. Cook, Zak D. J. Griffith et al. ALD-R491 regulates vimentin filament stability and solubility, cell contractile force, cell migration speed and directionality Frontiers in Cell and Developmental Biology 2022-11-22 [PMID: 36483676]
• MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus Elife, 2022-11-07;11(0):. 2022-11-07 [PMID: 36341713] (IHC, Mouse)
• O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 2022-09-28 [PMID: 36171203]
• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human)
• C Rodriguez-, N Kale, MJ Carlini, N Shrivastav, AA Rodrigues, B Khalil, JJ Bravo-Cord, M Alexander, J Ji, Y Hong, F Behbod, MS Sosa NR2F1 is a barrier to dissemination of early stage breast cancer cells Cancer Research, 2022-06-15;0(0):. 2022-06-15 [PMID: 35471456] (IF, Mouse)
• MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022-02-15;38(7):110379. 2022-02-15 [PMID: 35172130] (IHC, Human)
• de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O'Shea E The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease. Elife, 2021-09-30;10(0):. 2021-09-30 [PMID: 34590578] (ICC, Human)
• M Cui, A Atmanli, MG Morales, W Tan, K Chen, X Xiao, L Xu, N Liu, R Bassel-Dub, EN Olson Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance Nature Communications, 2021-09-06;12(1):5270. 2021-09-06 [PMID: 34489413] (IHC, Mouse)
• Coquand L, Victoria GS, Tata A et al. CAMSAPs organize an acentrosomal microtubule network from basal varicosities in radial glial cells The Journal of cell biology 8/2/2021 [PMID: 34019079]
• GT Park, JW Yoon, SB Yoo, YC Song, P Song, HK Kim, J Han, SJ Bae, KT Ha, NP Mishchenko, SA Fedoreyev, VA Stonik, MB Kim, JH Kim Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma Marine Drugs, 2021-04-23;19(5):. 2021-04-23 [PMID: 33922418] (IHC, Mouse)
• LA Tashireva, OE Savelieva, ES Grigoryeva, YV Nikitin, EV Denisov, SV Vtorushin, MV Zavyalova, NV Cherdyntse, VM Perelmuter Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients International Journal of Molecular Sciences, 2021-03-02;22(5):. 2021-03-02 [PMID: 33801519] (Flow Cytometry, Human)
• D Yamakawa, D Katoh, K Kasahara, T Shiromizu, M Matsuyama, C Matsuda, Y Maeno, M Watanabe, Y Nishimura, M Inagaki Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis Cell Reports, 2021-03-09;34(10):108817. 2021-03-09 [PMID: 33691104] (ICC, IHC, Mouse)
• SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-01-11;12(1):262. 2021-01-11 [PMID: 33431859] (IHC, Human)
• Kui Li, Zheng Zhou, Ju Li, Rui Xiang miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1 Technol Cancer Res Treat 2020-12-17 [PMID: 33327874]
• Z Wang, M Cui, AM Shah, W Tan, N Liu, R Bassel-Dub, EN Olson Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution Cell Reports, 2020-12-08;33(10):108472. 2020-12-08 [PMID: 33296652] (ICC, Human)
• P Saikia, JS Crabb, LL Dibbin, MJ Juszczak, B Willard, GF Jang, TM Shiju, JW Crabb, SE Wilson Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea Sci Rep, 2020-10-07;10(1):16717. 2020-10-07 [PMID: 33028893] (ICC, Rabbit)
• Matthew D Lauver, Daniel J Goetschius, Colleen S Netherby-Winslow, Katelyn N Ayers, Ge Jin, Daniel G Haas et al. Antibody escape by polyomavirus capsid mutation facilitates neurovirulence eLife 2020-09-17 [PMID: 32940605]
• Pino Bordignon, Giulia Bottoni, Xiaoying Xu, Alma S. Popescu, Zinnia Truan, Emmanuella Guenova et al. Dualism of FGF and TGF-beta Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant Cell Reports 2019-08-27 [PMID: 31461652]
• A Lopresti, F Malergue, F Bertucci, ML Liberatosc, S Garnier, Q DaCosta, P Finetti, M Gilabert, JL Raoul, D Birnbaum, C Acquaviva, E Mamessier Sensitive and easy screening for circulating tumor cells by flow cytometry JCI Insight, 2019-06-13;5(0):. 2019-06-13 [PMID: 31194699] (Flow Cytometry, Human)
• Zsuzsanna Szvicsek, Ádám Oszvald, Lili Szabó, Gyöngyvér Orsolya Sándor, Andrea Kelemen, András Áron Soós et al. Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors Cellular and Molecular Life Sciences 2019-06-01 [PMID: 31028424] (Immunohistochemistry, Human)
• S Wichaiyo, S Lax, SJ Montague, Z Li, B Grygielska, JA Pike, EJ Haining, A Brill, SP Watson, J Rayes Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice Haematologica, 2019-02-07;0(0):. 2019-02-07 [PMID: 30733265] (IHC-P, Mouse)
• Liu PH, Shah RB, Li Y et al. An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy Nat. Cell Biol. 2/1/2019 [PMID: 30664786]
• EM Langer, BL Allen-Pete, SM King, ND Kendsersky, MA Turnidge, GM Kuziel, R Riggers, R Samatham, TS Amery, SL Jacques, BC Sheppard, JE Korkola, JL Muschler, G Thibault, YH Chang, JW Gray, SC Presnell, DG Nguyen, RC Sears Modeling Tumor Phenotypes In�Vitro with Three-Dimensional Bioprinting Cell Rep, 2019-01-15;26(3):608-623.e6. 1/15/2019 [PMID: 30650355]
• Alexandra Pietraszkiewicz, Christopher Hampton, Sonny Caplash, Ling Lei, Yassemi Capetanaki, Gauri Tadvalkar et al. Desmin deficiency is not sufficient to prevent corneal fibrosis Experimental Eye Research 2019-03-01 [PMID: 30590024]
• CK Cajigas-Du, SR Martinez, L Woods-Burn, AM Durán, S Roy, A Basu, JA Ramirez, GL Ortiz-Hern, L Ríos-Colón, E Chirshev, ES Sanchez-He, U Soto, C Greco, C Boucheix, X Chen, J Unternaehr, C Wang, CA Casiano RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance Oncotarget, 2018-07-13;9(54):30363-30384. 2018-07-13 [PMID: 30100995] (Western Blot, Human)
• John M. Kane, Anthony Magliocco, Qiang Zhang, Dian Wang, Alex Klimowicz, Jonathan Harris et al. Correlation of High-Risk Soft Tissue Sarcoma Biomarker Expression Patterns with Outcome following Neoadjuvant Chemoradiation Sarcoma 2018-02-28 [PMID: 29681762]
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Review for Vimentin Antibody (MAB2105) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Sam Connell IHC-Fr Human 02/22/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Cultured Human Keratinocytes
• Species: Human
• Lot: UUQ0219011

Bioinformatics

• Gene Symbol: VIM
• Uniprot:
  • P08670()