VPS34 Antibody

Western Blot: VPS34 Antibody [NBP1-32545] - Non-transfected ( -) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [C1C3].

Western Blot: VPS34 Antibody [NBP1-32545] - (30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000

Product Discontinued

VPS34 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human VPS34. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Porcine (100%), Bovine (100%), Zebrafish (91%), Canine (100%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIK3C3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for VPS34 Antibody

• EC 2.7.1
• EC 2.7.1.137
• hVps34
• MGC61518
• phosphatidylinositol 3-kinase catalytic subunit type 3
• Phosphatidylinositol 3-kinase p100 subunit
• Phosphoinositide-3-kinase class 3
• phosphoinositide-3-kinase, class 3
• PI 3-Kinase C3
• PI3K type 3
• PI3-kinase type 3
• PIK3C3
• PtdIns-3-kinase type 3
• VPS34

Background

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for VPS34 Antibody (NBP1-32545). (Showing 1 - 1 of 1 FAQ).

1. Hi, could you tell us, what the lower band in the WB picture of the PIK3c3 antibody (at ~70kDA) is?
   • The lower band at ~75kDa that is observed in the WB has not been identified as of yet. I checked with our lab team and they have confirmed that they have observed this on numerous occasions however, the origin has not been determined. The PIK3C3 band that is the one at ~120kDa.

Bioinformatics

• Gene Symbol: PIK3C3