Wee1 Antibody

Western Blot: Wee1 Antibody [NBP3-04349] - Analysis of extracts of Jurkat cells, using Wee1 antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Discontinued

Wee1 Antibody Summary

• Immunogen: A synthetic peptide of human Wee1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WEE1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Wee1 Antibody

• DKFZp686I18166
• EC 2.7.10.2
• FLJ16446
• WEE1 homolog (S. pombe)
• Wee1
• wee1+ (S. pombe) homolog
• WEE1+ homolog
• Wee1A kinase
• WEE1A
• WEE1hu
• wee1-like protein kinase

Background

Wee1 encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WEE1