Western Blot Loading Control Antibody Pack

Product Discontinued

Western Blot Loading Control Antibody Pack Summary

• Immunogen: beta Actin: 50 ug: WB: H, M, R: Purified
  beta Tubulin:50 ug: WB: Bo, Ch, D, H, M, Mk, R, Xe: Purified
  GAPDH : 50 ug: WB: Ca, Chp, Dros, H, M, Mq, Pi, R: Purified
  Histone H2B: 50 ug: WB: Ch, D, Dros, H, M, Ma, R, Zf: Purified
  Anti-Rabbit Ig HRP Secondary: 50 ul: WB: Ra: Purified
  Bo: Bovine Ca:Cat Ch:Chicken Chp:Chimpanzee D:Dog Dros:Drosophila H:Human M:Mouse Ma:Mammals Mk:Monkey Mq:Macaque R:Rat Xe:Xenopus Zf:Zebrafish
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: The Western Blot Loading Control Sampler Kit is a cost-effective tool containing a panel of four antibodies specific to proteins frequently used for protein normalization, plus appropriate secondary reagent. Sufficient quantity of each antibody is included for several mini-blots; see individual datasheets for additional information on species reactivity and suggested dilutions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
• Purity: Protein G purified

Background

Genes that are constitutively and stably expressed at high levels in almost all tissues and cells are often referred to as housekeeping genes. Housekeeping proteins such as GAPDH, beta Actin, beta Tubulin, or Histone H2B (nuclear fractions) are useful as loading controls for western blots and protein normalization. Increasingly, researchers are making use of specific antibodies to housekeeping proteins to standardize and compare levels of protein. However, studies examining gene transcript and protein levels have shown that expression levels of housekeeping genes can vary. Collectively the results indicate that some housekeeping proteins may be better suited than others for use as loading controls depending on the model system used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for Western Blot Loading Control Antibody Pack (NBP2-25090)(1)

Publication using NBP2-25090

• Rajaraman G, Murthi P, Brennecke SP, Kalionis B. Homeobox gene HLX is a regulator of HGF/c-met-mediated migration of human trophoblast-derived cell lines. Biol Reprod. 2010-10-01 [PMID: 20554918] (WB, Human)
  
  Details:
  Product cited: Western Blot Loading Control Kit (IMG-6166K); GAPDH (IMG-5143, component) was used in WB in Human SGHPL-4 (trophoblast) and HTR-8/SVneo cells, Fig 7. Matrix metalloproteinases -8, -9, and -12 in smokers and patients with Stage 0 COPD. Illum