![Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - 100ng of each antigen was used for SDS-PAGE, in non-reduced form. Proteins were transferred using Transblot for 7 minutes at 25V. 5% dry milk in PBS-T was used as blocking buffer and dilution buffer for antibodies. Primary antibodies are given below, and goat anti-mouse-IgG-HRP secondary antibody was used at 1:1000. All steps were carried out for 1h at room temperature with gentle rocking. KPL Membrane TMB was used for detection. Development time 30 seconds.](http://images.novusbio.com/fullsize/Zika-Virus-VLP-Antibody-IG3-1-A12-G9-Western-Blot-NBP3-14850-img0001.jpg "Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - 100ng of each antigen was used for SDS-PAGE, in non-reduced form. Proteins were transferred using Transblot for 7 minutes at 25V. 5% dry milk in PBS-T was used as blocking buffer and dilution buffer for antibodies. Primary antibodies are given below, and goat anti-mouse-IgG-HRP secondary antibody was used at 1:1000. All steps were carried out for 1h at room temperature with gentle rocking. KPL Membrane TMB was used for detection. Development time 30 seconds.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA |
| Clone | IG3-1-A12-G9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Addition of 0.09% w/v sodium azide is recommended for long term storage at +4C. |
| Immunogen | Recombinant Zika virus VLPs (comprising envelope, pre-membrane and membrane proteins), produced in HEK293 cells |
| Specificity | This antibody (isotype IgG1 kappa) recognizes the Zika virus VLP comprising Envelope, Pre-membrane and Membrane proteins.
Antibody also recognises Envelope proteins from Dengue virus, Japanese Encephalitis virus and West Nile virus in ELISA assay. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Suitable for use in direct ELISA and Western blot (non-reduced). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS pH 7.4 |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antigen down ELISA using Zika virus VLPs. Antibody also recognises Dengue virus and Chikungunya virus VLPs.](http://images.novusbio.com/fullsize/Zika-Virus-VLP-Antibody-IG3-1-A12-G9-ELISA-NBP3-14850-img0002.jpg "ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antigen down ELISA using Zika virus VLPs. Antibody also recognises Dengue virus and Chikungunya virus VLPs.")
![ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antibody recognises Zika virus envelope protein and envelope proteins from Dengue virus (produced in insect and mammalian cells), Japanese Encephalitis virus and West Nile virus.](http://images.novusbio.com/fullsize/Zika-Virus-VLP-Antibody-IG3-1-A12-G9-ELISA-NBP3-14850-img0003.jpg "ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antibody recognises Zika virus envelope protein and envelope proteins from Dengue virus (produced in insect and mammalian cells), Japanese Encephalitis virus and West Nile virus.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")