Zyxin Antibody (CL2502) - BSA Free

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in U251 cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human kidney shows moderate to strong cytoplasmic and membranous positivity in cells in glomeruli and in tubules.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in HeLa cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human cell line A431 showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in MCF7 cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in U2OS cell line with Anti-ZYX monoclonal antibody, showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human endometrium shows strong cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human cerebral cortex shows no positivity in neuropil as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL2502
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Zyxin Antibody (CL2502) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q15942, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZYX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Zyxin Recombinant Protein Antigen (NBP2-36768PEP)

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (84%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Zyxin Antibody (CL2502) - BSA Free

• CTCL tumor antigen se14-3
• CTCL-associated antigen se14-3
• cutaneous T-cell lymphoma associated antigen se14-3
• Cutaneous T-cell lymphoma-associated antigen se14-3
• KIAA1125
• MGC31836
• predicted protein of HQ2893
• PRKCBP1
• PRO2893
• protein kinase C-binding protein 1
• Rack7
• RACK7protein kinase C binding protein 1
• zinc finger MYND domain containing protein 8
• Zinc finger MYND domain-containing protein 8
• zinc finger, MYND-type containing 8
• Zyx
• Zyxin 2
• Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZYX
• Uniprot:
  • Q15942()