HRP Conjugated Secondary Antibodies for Western Blot
A labeled secondary antibody is required to detect and visualize specific proteins in western blot analysis. The quality of western blot data can be compromised by the use of a low-quality secondary antibody. HRP-conjugated secondary antibodies from Novus are specific, sensitive, and optimized for western blot analysis. With secondary antibodies from over 25 different species and cited over 150 times in peer-reviewed publications, finding an HRP conjugated secondary antibody for your experiment should be simple.
Chemiluminescent detection using an HRP conjugated secondary antibody:
Western Blot: Rabbit Anti-Goat IgG Secondary Antibody [HRP] [HAF008] staining of brain lysate with Goat Anti-Syntaxin 1A [AF7237]
Western Blot: Goat Anti-Rabbit IgG Antibody [HRP] [HAF008] staining of cell line lysates with Rabbit Anti-Human/Mouse/Rat HSP60 [AF1800]
Secondary Antibody Handbook This guide provides an introduction to antibody structure and types, secondary antibody nomenclature, and technical information and tips to choose the best secondary antibody for your experiment.
Antibody Labeling Kits Don't want to use a secondary? Directly label nearly any primary antibody to a fluorescent, biotin, or enzyme label in just 30 seconds hands-on time with antibody labeling kits.
Understanding Direct vs. Indirect Detection Understand how indirect detection using a secondary antibody can increase assay sensitivity and make your multiple labeling experiment simpler.
Multicolor Panel Builder Use our tool to assist in antibody selection and to build multiplex panels for flow cytometry experiments. Our tool will help you choose the best combinations of antibodies.
