Loading Controls Handbook

Related Links

Western blot protocol

HRP Conjugated Secondaries

Western blot Troubleshooting

Western blot video protocol

This Loading Control Handbook will provide technical guidance on choosing and optimizing loading control proteins, as well as step-by-step instructions on how to use loading controls to normalize your Western blot data.

Read more about:

• Top loading controls based on target protein localization
• How to calculate relative target protein expression using a loading control
• Optimizing your loading control to generate accurate expression data
• Protocols to probe loading control expression

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