Are you a Western Blot expert? Test your Western knowledge with our crossword puzzle! The answers are at the bottom of the page.
Across
1. Housekeeping gene
4. Use an anti-mouse secondary antibody if primary is raised in _______
7. Consider ________ as the most important factor when selecting a secondary antibody
8. The most commonly used enzyme in Western blotting
10. Bromophenol blue is a small _____ dye
12. Most common gel condition
13. Recommended lysis buffer for membrane-bound proteins
15. Biochemical assay that measures the total amount of sample protein
Down
2. Use a _____ secondary antibody to prevent non-specific binding
3. Protein migration was too fast during electrophoresis, causing this effect
5. ______ is added to sample loading buffer in order to increase sample density
6. Reducing agent
9. Blocking agent not suitable for phosphorylated protein detection
11. The nuclear loading control, _________, is expressed in actively proliferating cells
14. Membrane that is better for low molecular weight proteins
