After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
Novus provides a variety of support products for research assays. These products include antigen retrieval buffers, knockout stem cells, g-linked agarose resins, purified human sera, and PBS tablets.
![pCL-Eco Retrovirus Packaging Vector [NBP2-29540]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29540-img0001.jpg "pCL-Eco Retrovirus Packaging Vector [NBP2-29540]")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.](http://images.novusbio.com/fullsize/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0004.jpg "Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit . Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).](http://images.novusbio.com/fullsize/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0005.jpg)
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Differentiated human macrophages were treated with or without 50 ng/mL Pam3CSK4 for 2 h, and the release of IL-6 in culture supernatants of cells was measured by ELISA kit.](http://images.novusbio.com/fullsize/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunofluorescence-NBP2-31156-img0002.jpg "Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.")
![Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunohistochemistry-NBP2-31156-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/DAPI-Solution-Immunocytochemistry-Immunofluorescence-NBP2-31156-img0001.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0003.jpg "MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.")
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. Cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of MALP2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: MALP2 specifically activated the TLR2/6-depedent NF-kB/SEAP reporter cells in a dose dependent manner. Note: HEK 293 cells endogenously express TLR6.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0004.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Differentiated human macrophages were treated with or without MALP-2 (100 ng/mL), and the release of IL-6 in culture supernatants of cells was measured by ELISA kit. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0008.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). Quadrant lines were drawn based on matched isotype controls.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Flow-Cytometry-NBA1-19349-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0007.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0009.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0010.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0011.jpg)
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0006.jpg "In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.")
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0007.jpg)
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0008.jpg)
![Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize/Imiquimod--TLR7-ligand-NBP2-26228-img0002.jpg "Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorter Assay kit.")
![CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](http://images.novusbio.com/fullsize/CpG-oligodeoxynucleotides-with-negative-control--TLR9-ligand-NBP2-26232-img0002.jpg "CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.")