| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49536SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33407-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93781-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22020-50ul | |||
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0010.jpg "Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Characterization of MPC1-/- in the RM-1 murine prostate cancer cells. Western blotting results of subcellular proteins from WT and MPC1-/- cells and corresponding densitometry histograms of ALT1 and Pyruvate Carboxylase. Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tublin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent and repeatable results. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0012.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse adipose tissue using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a specific staining of the fat cells in the tested section.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Pyruvate Carboxylase at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism. PLoS Pathog (2011)](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Knockdown-Validated-NBP1-49536-img0011.jpg)
![Western Blot: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-33407-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33407-img0004.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human liver and lymph node tissues. Corresponding PC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human cerebellum shows strong granular cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0009.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0010.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-278202415295530.jpg "Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32723) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29820241420961.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunocytochemistry analysis of PC-12 cells labeling PBC with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29202410334022.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32723, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-392024884671.jpg)
![Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp2-93781_rabbit-polyclonal-pyruvate-carboxylase-antibody-5220251931355.jpg "Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.")
![Immunoprecipitation: Pyruvate Carboxylase Antibody [NBP2-93781] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Pyruvate Carboxylase antibody (NBP2-93781). Western blot was performed from the immunoprecipitate using Pyruvate Carboxylase antibody (NBP2-93781) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunoprecipitation-NBP2-93781-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-93781] - Analysis of U2OS cells using Pyruvate Carboxylase . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93781-img0001.jpg)
![Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55361-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis in human cell lines HeLa and U-251M. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-55361-img0003.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-278202416331212.jpg "Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-2982024142096.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (SR1128) [NBP3-22020] - Western blot analysis using NBP3-22020 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22020_rabbit-pyruvate-carboxylase-mab-sr1128-57202316494259.jpg "Western Blot: Pyruvate Carboxylase Antibody (SR1128) [NBP3-22020] - Western blot analysis using NBP3-22020 in HepG2 cell lysate.")
![Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726] - Western blot analysis of extracts of various cell lines, using Pyruvate Carboxylase Rabbit mAb (NBP3-16726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-10S0V1-Western-Blot-NBP3-16726-img0001.jpg "Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726] - Western blot analysis of extracts of various cell lines, using Pyruvate Carboxylase Rabbit mAb (NBP3-16726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Pyruvate Carboxylase Overexpression Lysate (Adult Normal) [NBP2-10894] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Carboxylase.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10894-img0001.jpg "Western Blot: Pyruvate Carboxylase Overexpression Lysate (Adult Normal) [NBP2-10894] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Carboxylase.")
![Western Blot: Pyruvate Carboxylase Overexpression Lysate (Adult Normal) [NBL1-14138] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Carboxylase.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14138-img0002.jpg "Western Blot: Pyruvate Carboxylase Overexpression Lysate (Adult Normal) [NBL1-14138] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Carboxylase.")
![ELISA: Human Pyruvate Carboxylase ELISA Kit (Colorimetric) [NBP3-40296] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40296_human-pyruvate-carboxylase-elisa-kit-colorimetric-2110202415302917.png "ELISA: Human Pyruvate Carboxylase ELISA Kit (Colorimetric) [NBP3-40296] - Standard Curve Reference")