AK2 Antibodies
We offer AK2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each AK2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AK2 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the AK2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AK2 polyclonal antibodies and browse our AK2 monoclonal antibody catalog.
Alternate Names for AK2 Antibodies
anti-AK2 antibody, anti-adenylate kinase 2 antibody, anti-adenylate kinase 2, mitochondrial antibody, anti-adenylate kinase isoenzyme 2, mitochondrial antibody, anti-ADK2 antibody, anti-AK 2 antibody, anti-ATP-AMP transphosphorylase 2 antibody, anti-EC 2.7.4 antibody, anti-EC 2.7.4.3 antibody
18 Results for "ak2" in Primary Antibodies
![Western Blot: AK2 Antibody [NBP1-33160] - Wild-type (WT) and AK2 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with AK2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP1-33160-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AK2 Antibody [NBP1-33160] - Wild-type (WT) and AK2 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with AK2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AK2 Antibody [NBP1-33160] - Analysis of methanol-fixed HeLa, using Adenylate kinase 2 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33160-img0007.jpg)
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP1-33160-img0008.jpg)
![Western Blot: AK2 Antibody [NBP1-33160] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP1-33160-img0005.jpg)
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP1-33160-img0004.jpg)
![Western Blot: AK2 Antibody [NBP1-86043] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP1-86043-img0005.jpg "Western Blot: AK2 Antibody [NBP1-86043] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-86043] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP1-86043-img0001.jpg)
![Western Blot: AK2 Antibody [NBP1-86043] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP1-86043-img0004.jpg)
![Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts of various cell lines, using AK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP2-92954-img0003.jpg "Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts of various cell lines, using AK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-92954] - Paraffin-embedded human gastric cancer using AK2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-92954-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: AK2 Antibody [NBP2-92954] - Analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/AK2-Antibody-Knockout-Validated-NBP2-92954-img0005.jpg)
![Western Blot: AK2 Antibody [NBP2-99896] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP2-99896-img0005.jpg "Western Blot: AK2 Antibody [NBP2-99896] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa")
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-99896] - Immunochemical staining of human AK2 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-99896-img0004.jpg)
![Immunohistochemistry-Paraffin: AK2 Antibody [NBP2-99896] - Immunochemical staining of human AK2 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AK2-Antibody-Immunohistochemistry-Paraffin-NBP2-99896-img0003.jpg)
![Immunoprecipitation: AK2 Antibody [NBP2-99896] - AK2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-AK2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AK2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa](http://images.novusbio.com/fullsize/AK2-Antibody-Immunoprecipitation-NBP2-99896-img0001.jpg)
![Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: NIH/3T3 cell lysate](http://images.novusbio.com/fullsize/AK2-Antibody-AT7E7-Western-Blot-NBP2-50575-img0002.jpg "Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: NIH/3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: AK2 Antibody (AT7E7) [NBP2-50575] - Analysis of AK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK2 antibody with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/AK2-Antibody-AT7E7-Immunofluorescence-NBP2-50575-img0003.jpg)
![Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The Tissue lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysate](http://images.novusbio.com/fullsize/AK2-Antibody-AT7E7-Western-Blot-NBP2-50575-img0001.jpg)
![Western Blot: AK2 Antibody (SR1416) [NBP3-22569] - Western blot analysis using NBP3-22569 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22569_rabbit-ak2-mab-sr1416-6720239533799.jpg "Western Blot: AK2 Antibody (SR1416) [NBP3-22569] - Western blot analysis using NBP3-22569 in HepG2 cell lysate.")
![Western Blot: AK2 Antibody (JE64-27) [NBP3-32013] - Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32013_rabbit-ak2-mab-je64-27-27820241514179.jpg "Western Blot: AK2 Antibody (JE64-27) [NBP3-32013] - Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: AK2 Antibody (JE64-27) [NBP3-32013] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AK2 antibody (NBP3-32013) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody AK2 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32013_rabbit-ak2-mab-je64-27-29820241435236.jpg)
![Western Blot: AK2 Antibody (025) [NBP2-90183] - Lane A: Mouse kidney tissue lysateLane B: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/AK2-Antibody-025-Western-Blot-NBP2-90183-img0003.jpg "Western Blot: AK2 Antibody (025) [NBP2-90183] - Lane A: Mouse kidney tissue lysateLane B: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa")
![Immunohistochemistry-Paraffin: AK2 Antibody (025) [NBP2-90183] - Staining of human AK2 in human kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/AK2-Antibody-025-Immunohistochemistry-Paraffin-NBP2-90183-img0002.jpg)
![Immunohistochemistry-Paraffin: AK2 Antibody (025) [NBP2-90183] - Staining of human AK2 in human liver with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/AK2-Antibody-025-Immunohistochemistry-Paraffin-NBP2-90183-img0001.jpg)
![Western Blot: AK2 Antibody [NBP1-54639] - HepG2 cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP1-54639-img0002.jpg "Western Blot: AK2 Antibody [NBP1-54639] - HepG2 cell lysate, concentration 2.5 ug/ml.")
![Western Blot: AK2 Antibody [NBP3-00075] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/AK2-Antibody-Western-Blot-NBP3-00075-img0002.jpg "Western Blot: AK2 Antibody [NBP3-00075] - Anti-AK2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: AK2 Antibody (009) [NBP2-90182] - Immunofluorescence staining of AK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human AK2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AK2-Antibody-009-Immunofluorescence-NBP2-90182-img0001.jpg "Immunocytochemistry/Immunofluorescence: AK2 Antibody (009) [NBP2-90182] - Immunofluorescence staining of AK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human AK2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).")
![ELISA: AK2 Antibody (3F1) [H00000204-M01] - Detection limit for recombinant GST tagged AK2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AK2-Antibody-3F1-ELISA-H00000204-M01-img0001.jpg "ELISA: AK2 Antibody (3F1) [H00000204-M01] - Detection limit for recombinant GST tagged AK2 is 1 ng/ml as a capture antibody.")