Biotin Antibodies
We offer Biotin Antibodies for use in common research applications: ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Biotin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Biotin Antibodies can be used in a variety of model species: Human. Use the list below to choose the Biotin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Biotin polyclonal antibodies and browse our Biotin monoclonal antibody catalog.
Alternate Names for Biotin Antibodies
anti-Biotin antibody, anti-Vitamin B4 antibody
17 Results for "biotin" in Primary Antibodies
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0005.jpg "Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.")
![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0004.jpg)
![ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.](http://images.novusbio.com/fullsize/Biotin-Antibody-ELISA-NB120-6643-img0001.jpg)
![ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Biotin Antibody-ELISA-NB120-6643-img0007.jpg)
![Western Blot: Biotin Antibody [NBP2-15589] - Western blot experiment using at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control. A: Non-biotinlated control. B: Biotinylated protein.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NBP2-15589-img0001.jpg "Western Blot: Biotin Antibody [NBP2-15589] - Western blot experiment using at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control. A: Non-biotinlated control. B: Biotinylated protein.")
![Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] - Western blot analysis of various lysates, using Biotin Rabbit mAb at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16062_rabbit-biotin-mab-7h10r8-522025125429.jpg "Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] - Western blot analysis of various lysates, using Biotin Rabbit mAb at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] - Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15606_rabbit-biotin-pab-5220251734516.jpg "Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] - Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.")
![ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).](http://images.novusbio.com/fullsize/nbp3-23510_mouse-biotin-mab-1d4-c5-792023121223.jpg "ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).")
