eNOS Antibodies
We offer eNOS Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each eNOS Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eNOS Antibodies can be used in a variety of model species: Amphibian, Bovine, Canine, Drosophila, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the eNOS Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eNOS polyclonal antibodies and browse our eNOS monoclonal antibody catalog.
Alternate Names for eNOS Antibodies
anti-eNOS antibody, anti-NOS3 antibody, anti-Constitutive NOS antibody, anti-EC 1.14.13.39 antibody, anti-ECNOS antibody, anti-endothelial antibody, anti-Endothelial NOS antibody, anti-eNOScNOS antibody, anti-nitric oxide synthase 3 (endothelial cell) antibody, anti-NOS type III antibody, anti-NOSIII antibody
14 Results for "enos" in Primary Antibodies
![Western Blot: eNOS Antibody - BSA Free [NB300-500] - Fractionated, compared to single exposure, radiation more profoundly suppressed TM and eNOS. Representative Western blot analysis and quantification of KLF2 (n = 5) and KLF4 (n = 3) levels in whole-cell lysates from nonirradiated (sham) and irradiated HUVECs 4 h and 24 h after exposure to either five fractions of 2 Gy (5 x 2 Gy) or single exposure to 10 Gy. Fractions delivered at 24-h intervals. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/eNOS Antibody - BSA Free-Western Blot-NB300-500-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: eNOS Antibody - BSA Free [NB300-500] - Fractionated, compared to single exposure, radiation more profoundly suppressed TM and eNOS. Representative Western blot analysis and quantification of KLF2 (n = 5) and KLF4 (n = 3) levels in whole-cell lysates from nonirradiated (sham) and irradiated HUVECs 4 h and 24 h after exposure to either five fractions of 2 Gy (5 x 2 Gy) or single exposure to 10 Gy. Fractions delivered at 24-h intervals. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/eNOS-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB300-500-img0006.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Staining of eNOS in human lung tissue.](http://images.novusbio.com/fullsize/eNOS-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB300-500-img0003.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/eNOS-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB300-500-img0004.jpg)
![Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500] - Staining of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group. Dark brown staining of indicates eNOS-positive cells. Magnification x400.](http://images.novusbio.com/fullsize/eNOS-Antibody---BSA-Free-Immunohistochemistry-NB300-500-img0005.jpg)
![Western Blot: eNOS Antibody - BSA Free [NB300-500] - Mevalonate pathway inhibitors reversed fractionated-radiation–induced suppression of KLF2 & its downstream target molecules. Representative Western blot analysis & quantification of KLF2, TM, & eNOS 4 h after exposure to five fractions of 2 Gy (a) in presence or absence of atorvastatin (1 μM) or GT3 (5 μM) & (b) in presence or absence of GGTi (10 μM) (n = 3). beta -actin served as a loading control. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *, p < 0.05; **, p < 0.01; ***, p < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-500_rabbit-polyclonal-enos-antibody-31020241534534.jpg)
![Western Blot: eNOS Antibody - BSA Free [NB300-500] - Upregulation of HMG-CoA reductase in shPLD2 cells decreases eNOS expression. (A) Representative western blot of HMG-CoA reductase in Scramble & shPLD2 cells. The regions of the Western blots containing the HMG-CoA reductase & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system (B) Quantification of western blots with HMG-CoA reductase normalized to actin (n = 4 experiments). (C) N-SIM microscopy of HMG-CoA reductase; Bar, 7.5 μM. (D) RT-PCR of HMG-CoA reductase in Scramble & shPLD2 cells (n = 3). (E) Western blot of eNOS after treatment with NFOT & Simvastatin. Representative image of 3 experiments. The regions of the Western blots (Suppl. Figure 2) containing the eNOS & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system. (F) Quantification of western blots with eNOS levels normalized to actin (n = 3). Comparisons without bars made to untreated Scramble values. (G) eNOS activity after treatment with Simvastatin (n = 7). Mean ± SEM; *p < 0.05; **p < 0.01; ***p ≤ 0.001; One-way ANOVA with Bonferroni’s Multiple Comparison Test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28831159), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-500_rabbit-polyclonal-enos-antibody-310202416212339.jpg)
![Western Blot: eNOS Antibody - BSA Free [NB300-500] - The human PLD2 polymorphism R172C does not alter eNOS signaling but does decrease caveolin-1 protein levels. Overexpression of HA-tagged hPLD2 (A) & HA-R172C-PLD2 (B) in HeLa cells, visualized using anti-HA immunofluorescent staining (green). Arrowhead, PLD2 localization in filopodia; chevron, in peripheral actin ruffles; *, in dorsal actin ruffles; arrow, in subcortical actin network. Bar, 7.5 μM. Representative image of multiple experiments. (C) Representative western blot of eNOS after transfection of HA-hPLD2 or HA-R172C-PLD2 into shPLD2 cells. The regions of the Western blots containing the eNOS & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system. (D) Quantification of western blotting with eNOS levels normalized to actin (n = 3). (E) eNOS activity as measured by nitrate formation (n = 7). Lane 1 vs 2, P = 0.0021; lane 2 vs 3, P = 0.00035; lane 2 vs 4, P = 0.00012. (F–I) N-SIM microscopy of plasma membrane eNOS & caveolin-1. Bar, 7.5 μM. Representative image of multiple Scramble or shPLD2 cells imaged. Cells in H & I were selected for imaging based on expression of HA-hPLD2 or HA-R172C-PLD2 as visualized by anti-HA immunofluorescence in a separate channel (not shown). Mean ± SEM; *p < 0.05; **p < 0.01; ***p ≤ 0.001; One-way ANOVA with Bonferroni’s Multiple Comparison Test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28831159), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-500_rabbit-polyclonal-enos-antibody-310202416212351.jpg)
![Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg)
![Western Blot: eNOS [p Ser1177] Antibody [NBP3-05432] - Analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05432) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/eNOS-[p-Ser1177]-Antibody-Western-Blot-NBP3-05432-img0004.jpg "Western Blot: eNOS [p Ser1177] Antibody [NBP3-05432] - Analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05432) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: eNOS [p Ser1177] Antibody [NBP3-05432] - Mouse pancreas using eNOS [p Ser1177] antibody (NBP3-05432) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/eNOS-[p-Ser1177]-Antibody-Immunohistochemistry-Paraffin-NBP3-05432-img0002.jpg)
![Western Blot: eNOS [p Ser1177] Antibody [NBP3-05432] - Analysis of extracts of SKOV3 cells, using eNOS antibody (NBP3-05432). SKOV3 cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/eNOS-[p-Ser1177]-Antibody-Western-Blot-NBP3-05432-img0003.jpg)
![Immunohistochemistry-Paraffin: eNOS [p Ser1177] Antibody [NBP3-05432] - Mouse spinal cord using eNOS [p Ser1177] antibody (NBP3-05432) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/eNOS-[p-Ser1177]-Antibody-Immunohistochemistry-Paraffin-NBP3-05432-img0001.jpg)
![Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-19920231856919.jpg "Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: eNOS Antibody (M221) [NBP3-23209] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-11920231633165.jpg)
![Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-1992023141014.jpg "Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: eNOS Antibody [NBP3-23210] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-19920231856933.jpg)
![Western Blot: eNOS [p Tyr657] Antibody [NBP3-23185] - Western blot analysis of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-eNOS monoclonal antibody (NBP3-23209; lanes 1 & 2), anti-eNOS (Tyr-657) phospho-specific antibody (NP4031; lanes 3 & 4), or anti-eNOS polyclonal antibody (NBP3-23210; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23185_rabbit-enos-p-tyr657-pab-119202316331612.jpg "Western Blot: eNOS [p Tyr657] Antibody [NBP3-23185] - Western blot analysis of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-eNOS monoclonal antibody (NBP3-23209; lanes 1 & 2), anti-eNOS (Tyr-657) phospho-specific antibody (NP4031; lanes 3 & 4), or anti-eNOS polyclonal antibody (NBP3-23210; lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: eNOS [p Tyr657] Antibody [NBP3-23185] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23185_rabbit-enos-p-tyr657-pab-19920231410130.jpg)
![Western Blot: eNOS [p Ser632] Antibody (M232) [NBP3-23181] - Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23181_mouse-enos-p-ser632-mab-m232-11920231665313.jpg "Western Blot: eNOS [p Ser632] Antibody (M232) [NBP3-23181] - Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).")
![Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23180_rabbit-enos-pab-19920231759442.jpg "Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).")
![Western Blot: eNOS [p Thr495] Antibody [NBP3-05410] - Western blot analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05410) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/eNOS [p Thr495] Antibody-Western Blot-NBP3-05410-img0001.jpg "Western Blot: eNOS [p Thr495] Antibody [NBP3-05410] - Western blot analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05410) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).](http://images.novusbio.com/fullsize/eNOS-[p-Ser116]-Antibody-Western-Blot-NBP2-29664-img0002.jpg "Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).")