His Tag Antibodies
His Tag, also known as histidine tag or polyhistidine tag, is a common epitope tag used for protein purification and detection. A His Tag typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a protein. Hexahistidine (His6/6-His) tag is the most common His Tag and has a theoretical molecular weight of 0.8 kDa. We offer antibodies to detect His Tag that have been cited in 8+ publications with multiple 5-star reviews. Browse our extensive catalog of recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and His Tag-conjugated antibodies. Our His-Tag antibodies are suitable for multiple validated research applications including CyTOF-ready, ELISA, Flow Cytometry, Fluorophore-linked Immunosorbent Assay, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, IP, Simple Western, and Western Blot. Our His Tag antibodies can be used in a variety of model species: Human, All Species, Non-species specific, Multi-species. Each His Tag antibody is fully covered by Novus’ Guarantee+. Choose the anti-His Tag antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for His Tag Antibodies
anti-His Tag antibody
22 Results for "his-tag" in Primary Antibodies
![Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Simple-Western-NB100-64768-img0002.jpg "Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0008.jpg)
![Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Flow-Cytometry-NB100-64768-img0007.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0012.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0013.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0001.jpg)
![Western Blot: His Tag Antibody (RM146) [NBP2-61482] - Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y, using NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Western-Blot-NBP2-61482-img0008.jpg "Western Blot: His Tag Antibody (RM146) [NBP2-61482] - Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y, using NBP2-61482.")
![Immunohistochemistry: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of naive HepG2 cells (Negative control) using anti-His-Tag antibody, NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Immunohistochemistry-NBP2-61482-img0004.jpg)
![Immunohistochemistry-Paraffin: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Immunohistochemistry-Paraffin-NBP2-61482-img0001.jpg)
![Simple Western: His Tag Antibody (RM146) [NBP2-61482] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Simple-Western-NBP2-61482-img0007.jpg)
![Immunoprecipitation: His Tag Antibody (RM146) [NBP2-61482] - Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by NBP2-61482, was blotted with NBP2-61482. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Immunoprecipitation-NBP2-61482-img0009.jpg)
![Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Simple-Western-NBP2-43536-img0009.jpg "Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (359) [NBP2-43536] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 6X His tag stained by 6X His tag antibody [359] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Immunocytochemistry-Immunofluorescence-NBP2-43536-img0013.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins. B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins. 15 % SDS-PAGE. 6x His antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Western-Blot-NBP2-43536-img0011.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - Analysis of cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 ug of 6X His tag antibody [359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [359] diluted at 1:1000.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-Western-Blot-NBP2-43536-img0003.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - A) 2 ug GST-recombinant protein B) 2 ug His-recombinant protein C) 1 ug His-recombinant protein D) 0.5 ug His-recombinant protein E) 0.1 ug His-recombinant protein. 12% SDS-PAGE 6x His antibody at 1:5000.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Western-Blot-NBP2-43536-img0008.jpg)
![ELISA: His Tag Antibody (359) [NBP2-43536] - 6X His tag antibody [359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/mL 6X His tag antibody [359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-ELISA-NBP2-43536-img0001.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis.Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12 % SDS-PAGE6x His antibody (NBP2-43536) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-91020242075922.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with His Tag antibody [GT359] (NBP2-43536) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-910202420104472.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0001.jpg "Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0012.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0021.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0013.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 ug/mL).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0014.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5 ug of per well. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scietific ArrayScan or ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0024.jpg)
![Western Blot: His Tag Antibody (RMH01) [NBP2-61478] - Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using NBP2-61478 at 0.2 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RMH01-Western-Blot-NBP2-61478-img0005.jpg "Western Blot: His Tag Antibody (RMH01) [NBP2-61478] - Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using NBP2-61478 at 0.2 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.")
![Simple Western: His Tag Antibody (RMH01) [NBP2-61478] - Human purified protein.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RMH01-Simple-Western-NBP2-61478-img0004.jpg)
![ELISA: His Tag Antibody (RMH01) [NBP2-61478] - A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of NBP2-61478 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RMH01-ELISA-NBP2-61478-img0002.jpg)
![ELISA: His Tag Antibody (RMH01) [NBP2-61478] - Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with different concentrations of chimeric human anti-His Tag antibody NBP2-61478 as the capture antibody. An anti-H3K36M rabbit monoclonal antibody was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RMH01-ELISA-NBP2-61478-img0006.jpg)
![Western Blot: His Tag Antibody (His17) [NB100-72978] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-His17-Western-Blot-NB100-72978-img0002.jpg "Western Blot: His Tag Antibody (His17) [NB100-72978] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.")
![Simple Western: His Tag Antibody (His17) [NB100-72978] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-His17-Simple-Western-NB100-72978-img0001.jpg)
![Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-3D5-Simple-Western-NBP2-52637-img0002.jpg "Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Western Blot: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-52637 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141228.png)
![Immunocytochemistry /Immunofluorescence: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP2-52637 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141211.png)
![Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.](http://images.novusbio.com/fullsize/His-Tag-Antibody-Western-Blot-NBP1-25939-img0001.jpg "Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.")
![Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-Western-Blot-NBP1-42781-img0020.jpg "Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.")
![ELISA: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100 ug/mL. Dilution series: 2-fold. Mid-point concentration: 200 ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-ELISA-NBP1-42781-img0021.jpg)
![Western Blot: His Tag Antibody (6HIS/6402R) - Azide and BSA Free [NBP3-08360] - Western blot analysis of Recombinant His-Tag CELA3B protein using His Tag Recombinant Rabbit Monoclonal 6HIS/6402R).](http://images.novusbio.com/fullsize/His-Tag-Antibody-6HIS-6402R-Azide-and-BSA-Free-Western-Blot-NBP3-08360-img0001.jpg "Western Blot: His Tag Antibody (6HIS/6402R) - Azide and BSA Free [NBP3-08360] - Western blot analysis of Recombinant His-Tag CELA3B protein using His Tag Recombinant Rabbit Monoclonal 6HIS/6402R).")
![Western Blot: His Tag Antibody (6HIS/6402R) [NBP3-07271] - Western blot analysis of Recombinant His-Tag CELA3B protein using His Tag Recombinant Rabbit Monoclonal 6HIS/6402R).](http://images.novusbio.com/fullsize/His-Tag-Antibody-6HIS-6402R-Western-Blot-NBP3-07271-img0001.jpg "Western Blot: His Tag Antibody (6HIS/6402R) [NBP3-07271] - Western blot analysis of Recombinant His-Tag CELA3B protein using His Tag Recombinant Rabbit Monoclonal 6HIS/6402R).")