NDP52 Antibodies
We offer NDP52 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each NDP52 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NDP52 Antibodies can be used in a variety of model species: Human, Monkey, Mouse. Use the list below to choose the NDP52 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NDP52 polyclonal antibodies and browse our NDP52 monoclonal antibody catalog.
Alternate Names for NDP52 Antibodies
anti-NDP52 antibody, anti-CALCOCO2 antibody, anti-Antigen nuclear dot 52 kDa protein antibody, anti-calcium binding and coiled-coil domain 2 antibody, anti-calcium-binding and coiled-coil domain-containing protein 2 antibody, anti-NDP52MGC17318 antibody, anti-Nuclear domain 10 protein 52 antibody, anti-Nuclear domain 10 protein NDP52 antibody, anti-Nuclear dot protein 52 antibody
14 Results for "ndp52" in Primary Antibodies
![Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (c) Representative immunoblots (from 3 individuals/group) and expression profile (n = 6/group) of BNIP3 and NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0007.jpg "Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (c) Representative immunoblots (from 3 individuals/group) and expression profile (n = 6/group) of BNIP3 and NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148) licensed under a CC-BY license.")
![Western Blot: NDP52 Antibody [H00010241-B01P] - CALCOCO2 MaxPab polyclonal antibody. Western Blot analysis of CALCOCO2 expression in human pancreas.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0003.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human liver.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0004.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in transfected 293T cell line by CALCOCO2 polyclonal antibody. Lane 1: CALCOCO2 transfected lysate(49.06 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0006.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human pancreas.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0005.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (a) Representative immunoblots (n = 1/group) & expression profile (n = 6/group) of LC3I, LC3II at d17 (Supplemental Fig. S8) & (b) representative immunoblots (n = 1/group) & expression profile (n = 6/group) of DNM1 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). (c) Representative immunoblots (from 3 individuals/group) & expression profile (n = 6/group) of BNIP3 & NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). *p < 0.05 vs Control. #p < 0.05 vs LFA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010241-b01p_mouse-polyclonal-ndp52-antibody-310202415304229.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining in human testis and pancreas tissues using anti-CALCOCO2 antibody. Corresponding CALCOCO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining in human testis and pancreas tissues using anti-CALCOCO2 antibody. Corresponding CALCOCO2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: NDP52 Antibody [NBP1-87872] - Analysis using Anti-CALCOCO2 antibody NBP1-87872 (A) shows similar pattern to independent antibody NBP1-87874 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87872-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP1-87872] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87872-img0012.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0023.jpg)
![Western Blot: NDP52 Antibody [NBP1-87872] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87872-img0013.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0009.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0021.jpg)
![Western Blot: NDP52 Antibody [NBP1-87874] - Analysis using Anti-CALCOCO2 antibody NBP1-87874 (A) shows similar pattern to independent antibody NBP1-87872 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87874-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NDP52 Antibody [NBP1-87874] - Analysis using Anti-CALCOCO2 antibody NBP1-87874 (A) shows similar pattern to independent antibody NBP1-87872 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP1-87874] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87874-img0006.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0020.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human gastrointestinal, kidney, pancreas and testis using Anti-NDP52 antibody NBP1-87874 (A) shows similar protein distribution across tissues to independent antibody NBP1-87873 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0015.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0017.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0019.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI.Scale bar= 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19499-img0010.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP2-19499-img0003.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0006.jpg)
![Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunoprecipitation-NBP2-19499-img0009.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19499_rabbit-polyclonal-ndp52-antibody-910202420134026.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [422].](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [422].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - NDP52 antibody (422) detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody (422) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0012.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0001.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [422]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HepG2 cells mock (left) and treated with 3 uM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [422]. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0011.jpg)
![Immunoprecipitation: NDP52 Antibody (422) [NBP2-43696] - Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 ug of NDP52 antibody [422]. Western blot analysis was performed using NDP52 antibody [422]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunoprecipitation-NBP2-43696-img0006.jpg)
![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0006.jpg "Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: NDP52 Antibody (4H5) [NBP2-03246] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-03246-img0005.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDP52 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0003.jpg)
![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0004.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Hela cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0001.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Jurkat cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0002.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human gastrointestinal, kidney, pancreas and testis using Anti-NDP52 antibody NBP1-87873 (A) shows similar protein distribution across tissues to independent antibody NBP1-87874 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0014.jpg)
![Western Blot: NDP52 Antibody [NBP1-87873] - Analysis in control (vector only transfected HEK293T lysate) and CALCOCO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87873-img0009.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0015.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0016.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0017.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (1813) [NBP2-43588] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [1813]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Immunocytochemistry-Immunofluorescence-NBP2-43588-img0006.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0001.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. The ACTB was used as an internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0005.jpg)
![Western Blot: NDP52 Antibody [NBP3-38417] - Western blot analysis of lysates from mouse pancreas, using NDP52 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38417_rabbit-ndp52-pab-1012202411421036.jpg "Western Blot: NDP52 Antibody [NBP3-38417] - Western blot analysis of lysates from mouse pancreas, using NDP52 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP3-38417] - Immunofluorescence analysis of PC-12 cells using NDP52 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38417_rabbit-ndp52-pab-311202516593615.jpg)
![Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636] - Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (NBP3-32636) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32636) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32636_rabbit-ndp52-mab-psh02-33-278202415212481.jpg "Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636] - Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (NBP3-32636) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32636) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: NDP52 Antibody (PSH02-33) [NBP3-32636] - Flow cytometric analysis of HeLa cells labeling NDP52. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32636, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32636_rabbit-ndp52-mab-psh02-33-29202416453247.jpg)
![Western Blot: NDP52 Antibody (5O8Y6) [NBP3-33327] - Western blot analysis of various lysates using NDP52 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33327_rabbit-ndp52-mab-5o8y6-912202415121386.jpg "Western Blot: NDP52 Antibody (5O8Y6) [NBP3-33327] - Western blot analysis of various lysates using NDP52 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: NDP52 Antibody (10Q0T10) [NBP3-33472] - Western blot analysis of various lysates, using NDP52 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33472_rabbit-ndp52-mab-10q0t10-101220241243917.jpg "Western Blot: NDP52 Antibody (10Q0T10) [NBP3-33472] - Western blot analysis of various lysates, using NDP52 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")