Nitrotyrosine Antibodies
We offer Nitrotyrosine Antibodies for use in common research applications: ELISA, Immunoassay, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Nitrotyrosine Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Nitrotyrosine Antibodies can be used in a variety of model species: Canine, Human, Mouse, Multi-Species, Rat. Use the list below to choose the Nitrotyrosine Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Nitrotyrosine polyclonal antibodies and browse our Nitrotyrosine monoclonal antibody catalog.
Alternate Names for Nitrotyrosine Antibodies
anti-Nitrotyrosine antibody
9 Results for "nitrotyrosine" in Primary Antibodies
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0018.jpg "Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.")
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0021.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0017.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0014.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0019.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0020.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0022.jpg)
![Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899] - Rat left ventricle embedded in OCT, flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat, fixed with ice-cold acetone, blocked using 2% BSA in PBS with 5% normal goat serum, followed by incubation with nitrotyrosine antibody at 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 at 1:250 dilution for 1hr at RT. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-Immunohistochemistry-Frozen-NB100-2899-img0001.jpg "Immunohistochemistry-Frozen: Nitrotyrosine Antibody [NB100-2899] - Rat left ventricle embedded in OCT, flash-frozen in liquid nitrogen and stored at -80C. 8 um sections cut using a cryostat, fixed with ice-cold acetone, blocked using 2% BSA in PBS with 5% normal goat serum, followed by incubation with nitrotyrosine antibody at 1:50 dilution overnight at 4C. Secondary antibody was goat anti-rabbit Alexa Fluor 488 at 1:250 dilution for 1hr at RT. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-EM-30-Immunohistochemistry-Frozen-NBP1-96130-img0001.jpg "Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).")
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-HM11-Immunohistochemistry-Paraffin-NB600-1024-img0003.jpg "Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).")
![Western Blot: Nitrotyrosine Antibody [NBP3-16015] - Western blot analysis of extracts of various cell lines, using Nitrotyrosine antibody (NBP3-16015) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate(3mM)for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-Western-Blot-NBP3-16015-img0001.jpg "Western Blot: Nitrotyrosine Antibody [NBP3-16015] - Western blot analysis of extracts of various cell lines, using Nitrotyrosine antibody (NBP3-16015) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate(3mM)for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Nitrotyrosine Antibody [NBP3-16015] - Western blot analysis of extracts of NIH/3T3 cells, using Nitrotyrosine antibody (NBP3-16015) at 1:1000 dilution.NIH3T3 cells were treated by Perxynitrate(3mM)for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-Western-Blot-NBP3-16015-img0002.jpg)
![Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.](http://images.novusbio.com/fullsize/nbp3-30876_mouse-nitrotyrosine-mab-2a12-811202420592158.jpg "Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.")