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NKX2.5 Antibodies

We offer NKX2.5 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each NKX2.5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NKX2.5 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat. Use the list below to choose the NKX2.5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NKX2.5 polyclonal antibodies and browse our NKX2.5 monoclonal antibody catalog.

Alternate Names for NKX2.5 Antibodies

anti-NKX2.5 antibody, anti-NKX2-5 antibody, anti-cardiac-specific homeo box antibody, anti-cardiac-specific homeobox 1 antibody, anti-Cardiac-specific homeobox antibody, anti-CHNG5 antibody, anti-CSX1 antibody, anti-CSXFLJ97195 antibody, anti-FLJ52202 antibody, anti-FLJ99536 antibody, anti-Homeobox protein CSX antibody, anti-Homeobox protein NK-2 homolog E antibody, anti-homeobox protein Nkx-2.5 antibody, anti-NK2 transcription factor related, locus 5 (Drosophila) antibody, anti-NKX2.5FLJ97166 antibody, anti-NKX2EFLJ97197 antibody, anti-NKX4-1 antibody, anti-tinman paralog antibody

7 Results for "nkx25" in Primary Antibodies

AF2444

NKX2.5 Antibody [Unconjugated]

(2 Reviews)

(14 Publications)

100 μg

AF2444

EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating doxycycline-dependent EOMES expression in a transgenic EOMESKO/E.TET-ON hESC line. b Typical yields of hESC-CMs (left, flow cytometry) and NKX2.5 expression (right, immunoblot) obtained with the two protocols (day 10). c Immunostainings 21 days after the initiation of EOMES induction. Weak perinuclear ANP staining is typical in overall MLC2v-positive hESC-CMs. Scale bars: 25 (top) and 50 µm (bottom). d Acceleration and slowdown of spontaneous beat rates in pCMs following exposure to 10 µM isoprenaline and 10 µM propranolol, respectively, on multielectrode arrays. e Microarray-based time course analysis comparing the indicated protocols and cell lines. RESCUE cells carry an inducible EOMES transgene on EOMESKO HuES6 background. Underlying data are from Supplementary Data 2 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Contextual requirements of EOMES-mediated CM programming. a DOX dose dependency of the EOMES TET-ON protocol using the WTE.TET-ON hESC line. Top panel: Immunostains at 1.5 wk. Scale bar: 100 µm. Bottom: Flow cytometry analysis. Numbers indicate average percentages of cTnT-positive CMs from 3–6 experiments per condition. b CM programming by EOMES necessitates suppression of autocrine WNT signaling from the third day of transgene induction (qPCR data, n = 2). c DOX dose-dependent CM programming using an independent WTE.TET-ON hiPSC line. The data shows immunostaining (scale bar: 100 µm), RT-qPCR (n = 4), and FACS analysis (n = 3) performed at 1 wk of differentiation. The asymmetrical shape of the DOX titration data with this line is in part due to the incomplete repression of SOX2 at 0.05 µg/ml, which caused overgrowth of the cultures with neural precursors (also see Supplementary Fig. 3f). Error bars: s.e.m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

EOMES knockout hESCs fail to differentiate into cardiomyocytes. a Immunoblot confirming EOMES expression and its absence in WT and KO cells, respectively, at the cardiac mesoderm stage of directed differentiation. b EOMES KO cells fail to express the early cardiomyocyte marker NKX2.5 following exposure to a directed differentiation protocol. Scale bar: 50 µm. c EOMES KO cells show a general failure in markedly upregulating essential pan-cardiac genes (qPCR data, n = 3; error bars: s.e.m.). For each gene, the expression level in the highest expressing sample (“max” = WT in all three cases) is set to 1. d Differentiation of EOMES KO hESCs under signaling factor-assisted, non-cardiac mesoderm/endoderm-permissive culture conditions. Top: EOMES KO cells fail to express alpha -fetoprotein following Activin A-assisted endodermal induction. Scale bar: 50 µm. Bottom: Non-cardiac mesodermal differentiation competence of EOMES KO hESCs. KO cells were differentiated using varied meso and endoderm-permissive induction conditions. The data denote transformed p-values of annotation terms enriched in gene sets upregulated in EOMES KO samples compared to undifferentiated hESCs or differentiated WT controls (cutoffs: 10 or 3-fold, respectively). Underlying data: Supplementary Data 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2444

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2444-SP

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MAB2444

NKX2.5 Antibody (259416)

(9 Publications)

100 μg

MAB2444

NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2444-SP

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NBP1-31558

NKX2.5 Antibody - BSA Free

(2 Publications)

100 ul

NBP1-31558

Immunocytochemistry/Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558] - Mouse heart. Nkx2.5 stained by Nkx2.5 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558] - Rat heart. Nkx2.5 stained by Nkx2.5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: NKX2.5 Antibody [NBP1-31558] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: NKX2.5 Antibody [NBP1-31558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - NKX2.5 antibody detects NKX2.5 protein at nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NKX2.5 stained by NKX2.5 antibody (NBP1-31558) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Western Blot: NKX2.5 Antibody [NBP1-31558] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-24675

NKX2.5 Antibody

0.1 mg

NBP2-24675

Western Blot: Nkx2.5 Antibody [NBP2-24675] - Western blot analysis of Nkx2.5 in human heart lysate using 1) prebleed and 2) NBP2-24675 at 1 ug/ml.

Immunohistochemistry-Paraffin: Nkx2.5 Antibody [NBP2-24675] - Analysis of NKX2-5 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-24675 (bottom left, right) at 5 ug/ml.

Reactivity:

Hu, Pm, Po, Bv, Pm, Eq, Fe, RM

Species Glossary

Reactivity: Hu, Pm, Po, Bv, Pm, Eq, Fe, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6493A

Alt Catalog# IMG-6493A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-51953

NKX2.5 Antibody

(2 Publications)

0.1 mg

NBP1-51953

Western Blot: NKX2.5 Antibody [NBP1-51953] - (1ug/ml) staining of Human (A), Mouse (B) and (2ug/ml) Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB24441

NKX2.5 Antibody (259415) [Unconjugated]

100 μg

MAB24441

Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB24441-SP

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BAF2444

NKX2.5 Antibody [Biotin]

50 μg

BAF2444

NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human NKX2.5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2444) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2444
Carrier Free	AF2444-SP

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