PML Protein Antibodies
We offer PML Protein Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each PML Protein Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PML Protein Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PML Protein Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PML Protein polyclonal antibodies and browse our PML Protein monoclonal antibody catalog.
Alternate Names for PML Protein Antibodies
anti-PML Protein antibody, anti-PML antibody, anti-MYLPP8675 antibody, anti-promyelocytic leukemia antibody, anti-Promyelocytic leukemia protein antibody, anti-RING finger protein 71 antibody, anti-RNF71probable transcription factor PML antibody, anti-TRIM19promyelocytic leukemia, inducer of antibody, anti-tripartite motif protein TRIM19 antibody, anti-Tripartite motif-containing protein 19 antibody
17 Results for "pml-protein" in Primary Antibodies
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0018.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.")
![Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-NB100-59787-img0017.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0016.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0020.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0015.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0006.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0014.jpg)
![Western Blot: PML Protein Antibody - BSA Free [NB100-59787] - PML-RARa is a HIF-alpha transcriptional co-activator.A HIF-alpha transactivation assays (A–D) with HRE-luciferase construct. Results are presented as Luciferase/Renilla ratio (mean ± s.e.m. of experiments performed in triplicate).B HEK-293 cells transfected with stable mutants of HIF-1 alpha or HIF-2 alpha & increasing concentrations of PML-RAR alpha .C HEK-293 cells transfected with a stable form of HIF-1 alpha along with PML-RAR alpha , PML or RAR alpha .D Wild-type & Pml−/− MEFs transfected with HIF-1 alpha & PML-RAR alpha . Asterisks indicate fold change induction of HIF-1 alpha -mediated transactivation upon PML-RAR alpha expression.E HEK-293 cells transfected with the indicated fusion genes & treated with CoCl2.F Co-immunoprecipitation of exogenous stable forms of HIF-1 alpha (left panel) & HIF-2 alpha (right panel) & PML-RAR alpha with a PML-directed antibody in HEK-293 cells. Of note, exogenously expressed PML-RAR alpha migrates very closely to endogenous PML.Co-immunoprecipitation of endogenous HIF-1 alpha & PML-RAR alpha with a PML-directed antibody in NB4 cells treated with CoCl2.Data information: All experiments were repeated at least twice.Source data are available for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24711541), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-59787_rabbit-pml-protein-pab-bsa-free-3102024168217.jpg)
![Immunohistochemistry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32832) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-298202414115433.jpg "Immunohistochemistry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32832) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PML Protein Antibody (PSH02-89) [NBP3-32832] - Immunocytochemistry analysis of MDA-MB-231 cells labeling PML Protein with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-29202410334037.jpg)
![Flow Cytometry: PML Protein Antibody (PSH02-89) [NBP3-32832] - Flow cytometric analysis of A431 cells labeling PML Protein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32832, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-29202416453264.jpg)
![Western Blot: PML Protein Antibody (PSH02-89) [NBP3-32832] - Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (NBP3-32832) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: K-562 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: MDA-MB-231 cell lysate (15 µg/Lane) Lane 6: A431 cell lysate (15 µg/Lane) Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32832_rabbit-pml-protein-mab-psh02-89-3920248453512.jpg)
![Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59787 and another PML antibody.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59788-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59787 and another PML antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-NB100-59788-img0005.jpg)
![Western Blot: PML Protein Antibody (PSH02-68) [NBP3-32831] - Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (NBP3-32831) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: A431 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32831) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32831_rabbit-pml-protein-mab-psh02-68-288202412442981.jpg "Western Blot: PML Protein Antibody (PSH02-68) [NBP3-32831] - Western blot analysis of PML Protein on different lysates with Rabbit anti-PML Protein antibody (NBP3-32831) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: A431 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 98 kDa Observed band size: 50-130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32831) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PML Protein Antibody (PSH02-68) [NBP3-32831] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PML Protein antibody (NBP3-32831) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32831) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32831_rabbit-pml-protein-mab-psh02-68-298202414115434.jpg)
![Immunocytochemistry/ Immunofluorescence: PML Protein Antibody (PSH02-68) [NBP3-32831] - Immunocytochemistry analysis of MDA-MB-231 cells labeling PML Protein with Rabbit anti-PML Protein antibody (NBP3-32831) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PML Protein antibody (NBP3-32831) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32831_rabbit-pml-protein-mab-psh02-68-29202410234237.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-87783-img0006.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.")
![Western Blot: PML Protein Antibody (SR1825) [NBP3-21728] - Western blot analysis using NBP3-21728 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21728_rabbit-pml-protein-mab-sr1825-5720231595513.jpg "Western Blot: PML Protein Antibody (SR1825) [NBP3-21728] - Western blot analysis using NBP3-21728 in 293 cell lysate.")
![Western Blot: PML Protein Antibody [NBP2-94515] - Analysis of extracts of various cell lines, using PML Protein . Exposure time: 3min.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-94515-img0001.jpg "Western Blot: PML Protein Antibody [NBP2-94515] - Analysis of extracts of various cell lines, using PML Protein . Exposure time: 3min.")
![Immunohistochemistry: PML Protein Antibody - BSA Free [PML Protein] - Immunohistochemistry analysis of paraffin-embedded human appendix using PML Protein Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94515_rabbit-polyclonal-pml-protein-antibody-5220251638039.jpg)
![Western Blot: PML Protein Antibody [NBP2-98823] - Anti-PML Protein rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-98823-img0003.jpg "Western Blot: PML Protein Antibody [NBP2-98823] - Anti-PML Protein rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98823] - Immunofluorescence staining of PML Protein in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98823-img0001.jpg)
![Immunoprecipitation: PML Protein Antibody [NBP2-98823] - PML Protein was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 4 ul anti-PML Protein rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-PML Protein rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunoprecipitation-NBP2-98823-img0002.jpg)
![Immunohistochemistry:[NBP3-30289]-Detection of human PML by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PML used at a dilution of 1: 1,000. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30289_rabbit-pml-protein-pab-231202511585595.jpg "Immunohistochemistry:[NBP3-30289]-Detection of human PML by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PML used at a dilution of 1: 1,000. Detection: DAB")
![Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP3-18686-img0001.jpg "Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.")
![ELISA: PML Protein Antibody [NBP3-18686] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PML Protein , crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.](http://images.novusbio.com/fullsize/PML Protein Antibody-ELISA-NBP3-18686-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98822-img0001.jpg "Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.")
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0003.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0002.jpg)
![Western Blot: PML Protein Antibody [NBP2-19872] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP2-19872-img0001.jpg "Western Blot: PML Protein Antibody [NBP2-19872] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.")
![Immunoprecipitation: PML Protein Antibody [NBP2-19872] - Immunoprecipitation of PML protein from A549 whole cell extracts using 5 ug of PML antibody [N1N2], N-term. Western blot analysis was performed using PML antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunoprecipitation-NBP2-19872-img0004.jpg)