RFP Antibodies
We offer RFP Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot. Each RFP Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RFP Antibodies can be used in a variety of model species: Human. Use the list below to choose the RFP Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RFP polyclonal antibodies and browse our RFP monoclonal antibody catalog.
Alternate Names for RFP Antibodies
anti-RFP antibody, anti-DSR antibody, anti-dsRedGene Symbol antibody, anti-Recombinant RFP Protein antibody, anti-Red Fluoroscent Protein antibody
8 Results for "rfp" in Primary Antibodies
![Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0025.jpg "Western Blot: RFP Antibody [NBP1-69962] - Western blot of RFP recombinant protein detected with RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Immunofluorescence Microscopy of RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue.Fixation: 4% PFA. Antigen retrieval: Heat.Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT.Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT.Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0019.jpg)
![Immunohistochemistry: RFP Antibody [NBP1-69962] - Immunohistochemistry of RFP antibody.Tissue: Mouse gut tissue in tomato transgenic mice.Fixation: formalin fixed paraffin embedded. Antigen retrieval: heat 5 min at high temp in 1X rodent decloaker. Primary antibody: RFP antibody at 1:200 for 1 h 30 min at RT.Secondary Antibody: HRP anti-rabbit.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunohistochemistry-NBP1-69962-img0026.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye 800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0008.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: RFP. Lane 2: Human IgG. Lane 3: Goat IgG. Lane 4: Mouse IgG. Load: 50ng per lane.Primary antibody: RFP antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT.Block Blocking Buffer for 30 min at RT.Predicted/Observed size: 27 kDa, 30 kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0021.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Marker.Lane 2: 50ng of RFP. Primary Antibody: Anti-RFP at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30mins at RT. Block: BlockOut Universal blocking buffer. Expect ~27kDa.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0022.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: RFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 30 kDa for RFP.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0023.jpg)
![Western Blot: RFP Antibody [NBP1-69962] - Western Blot of RFP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: RFP/HEK293T WCL [0.05/10ug] [+]. Lane 3: HEK293T Whole Cell Lysate [-]. Lane 4: RFP/NIH/3T3 Whole Cell Lysate [0.05/10ug] [+]. Lane 5: NIH/3T3 Whole Cell Lysate [-]. Lane 6: RFP/PC-12 Whole Cell Lysate [0.05/10ug] [+]. Lane 7: PC-12 Whole Cell Lysate [-]. Primary Antibody: Anti-RFP at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase at 1:70,000 at RT for 30 mins. Predicted/Observed MW: ~27kDa. Exposure: 2 sec.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-69962-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom/+ mice. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Primary antibody: RFP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse lung tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: SPC+ cells are RFP positive in red.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-69962] - Rabbit Anti-RFP antibody. Tissue: (10X) Mouse E14.5 embryo heart tissue. Fixation: 4% PFA. Antigen retrieval: Heat. Primary antibody: Anti-RFP antibody at 1:50 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:250 for 1 hr at RT. Staining: cardiac cells are RFP positive in red in tomato transgenic mice.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69962-img0003.jpg)
![CyTOF-ready: RFP Antibody [NBP1-69962] - Analysis of RFP antibody on human tissue. RFP-expressing cells (red), nuclei (blue). Scale, 50 um. Image from verified customer review](http://images.novusbio.com/fullsize/RFP-Antibody-CyTOF-ready-NBP1-69962-img0016.jpg)
![ELISA: RFP Antibody [NBP1-69962] - ELISA Results of RFP antibody tested against purified RFP protein. Each well was coated in duplicate with 1.0 ug of RFP [red], Human IgG [green], Mouse IgG [blue], Rat IgG [purple]. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% Fish Gel in PBS Blocking Buffer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate. Results show RFP titer >75,000, Human, Mouse, Rat IgG titers <100.](http://images.novusbio.com/fullsize/RFP-Antibody-ELISA-NBP1-69962-img0027.jpg)
![Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-97371-img0001.jpg "Western Blot: RFP Antibody [NBP1-97371] - Antibody was added at 1.0ug/mL at RT for 2 hours. IRDye800(R) anti-Chicken was added at 1:20,000 at RT for 45 minutes.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RFP Antibody [NBP1-97371]](http://images.novusbio.com/fullsize/RFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97371-img0002.jpg)
![Western Blot: RFP Antibody (1610) [NBP2-43713] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody [GT1610] diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RFP-Antibody-1610-Western-Blot-NBP2-43713-img0004.jpg "Western Blot: RFP Antibody (1610) [NBP2-43713] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody [GT1610] diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody (1610) [NBP2-43713] - Analysis of RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP-recombinant protein stained by RFP-tag antibody diluted at 1:2000. Red: RFP is expressed in the tranfected cell . Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RFP-Antibody-1610-Immunocytochemistry-Immunofluorescence-NBP2-43713-img0001.jpg)
![Immunoprecipitation: RFP Antibody (1610) [NBP2-43713] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RFP-Antibody-1610-Immunoprecipitation-NBP2-43713-img0002.jpg)
![Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP2-19302-img0010.jpg "Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunoprecipitation-NBP2-19302-img0003.jpg)
![Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19302_rabbit-polyclonal-rfp-antibody-9102024207593.jpg)
![Immunocytochemistry/ Immunofluorescence: RFP Antibody [NBP2-19302] - RFP antibody detects RFP protein at by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RFP protein stained by RFP antibody (NBP2-19302) diluted at 1:5000.Red: RFP is expressed in the RFP-fusion protein tranfected cell.Blue: Hoechst 33342 staining.The cell of merger panel with no RFP expressed as a negative control.](http://images.novusbio.com/fullsize/nbp2-19302_rabbit-polyclonal-rfp-antibody-910202419593595.jpg)
![ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-ELISA-NBP3-12167-img0001.jpg "ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.")
![Dot Blot: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - Row A: Recombinant RFP protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase at 1:5000 for 30 mins at RT. Block: BlockOut Buffer.. Exposure: 10 seconds.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-Dot-Blot-NBP3-12167-img0002.jpg)
![Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25634_rabbit-rfp-mab-hl2028-azide-and-bsa-free-2120249415930.jpg "Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis. Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP tagged protein stained by RFP-tag antibody (NBP3-25634) diluted at 500. Red: RFP tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25634_rabbit-rfp-mab-hl2028-azide-and-bsa-free-2120249351310.jpg)
![Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/RFP-Antibody-1433-Western-Blot-NBP2-43580-img0002.jpg "Western Blot: RFP Antibody (1433) [NBP2-43580] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug 293T whole cell extract expressing GFP-tagged protein C. 30 ug 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody dilution: 1:10000.")
![Immunoprecipitation: RFP Antibody (1433) [NBP2-43580] - Analysis of 400 ug cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 ug of RFP tag antibody 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody diluted at 1:1000. EasyBlot anti-nouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RFP-Antibody-1433-Immunoprecipitation-NBP2-43580-img0001.jpg)
![Western Blot: RFP Antibody [NBP1-45840] - Analysis with recombinant RFP. Lane 1: 10 ng; Lane 2: 50 ng; Lane 3: 250 ng; Lane 4: 1 ug.](http://images.novusbio.com/fullsize/RFP-Antibody-Western-Blot-NBP1-45840-img0002.jpg "Western Blot: RFP Antibody [NBP1-45840] - Analysis with recombinant RFP. Lane 1: 10 ng; Lane 2: 50 ng; Lane 3: 250 ng; Lane 4: 1 ug.")