RUNX2/CBFA1 Antibodies
RUNX2/CBFA1, also known by the names of runt-related transcription factor 2 and core-binding factor subunit alpha-1, has a theoretical molecular weight of 56 kDa. RUNX2/CBFA1 is a transcription factor that has a crucial role in osteoblast differentiation and bone development. We offer antibodies to detect RUNX2/CBFA1 that have been cited in 48+ publications. Browse our extensive catalog of monoclonal, polyclonal, and RUNX2/CBFA1-conjugated antibodies. Validated research applications for RUNX2/CBFA1 antibodies include Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Sandwich ELISA, and Western Blot. Our RUNX2/CBFA1 antibodies can be used in a variety of model species including Human, Mouse, Rat, Canine, Chicken, Equine, and Primate. Each RUNX2/CBFA1 antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Choose the anti-RUNX2/CBFA1 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for RUNX2/CBFA1 Antibodies
anti-RUNX2/CBFA1 antibody, anti-RUNX2 antibody, anti-Acute myeloid leukemia 3 protein antibody, anti-AML3CCD antibody, anti-CBFA1MGC120022 antibody, anti-Core-binding factor subunit alpha-1 antibody, anti-MGC120023 antibody, anti-PEA2-alpha A antibody, anti-PEBP2-alpha A antibody, anti-PEBP2A antibody, anti-PEBP2A1CCD1 antibody, anti-PEBP2aA1 antibody, anti-runt domain, alpha subunit 1 antibody, anti-runt-related transcription factor 2 antibody, anti-SL3-3 enhancer factor 1 alpha A subunit antibody, anti-SL3/AKV core-binding factor alpha A subunit antibody
15 Results for "runx2-cbfa1" in Primary Antibodies
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31388010/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0009.jpg "Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31388010/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with RUNX2/CBFA1 Antibody (NBP1-77461) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77461-img0010.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in chondrogenic pellets or in cell monolayer in control medium: levels of expression of osteoblast markers (1), chondrocyte markers (2) and tenocyte markers (3) (N = 3) assessed by qPCR.* =0.05. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31561454/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0008.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-NBP1-77461-img0006.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - Histological characterization of rotator cuff calcifications (N = 5). (A) Calcific deposits assessed by micro-computed tomography. (B) HE (hematoxylin & eosin) staining of decalcified samples (1 image for each patient). (C) Von Kossa staining on not decalcified samples & characterization of the fibrocartilaginous area with alcian blue staining, SOFG (Safranin O/Fast Green) staining, & immunohistochemical staining for COL2 (brown) with Gill hematoxylin counterstain. (D,E) Representative immunohistochemical staining for ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), TNAP (tissue non-specific alkaline phosphatase), Runx2, Sox9, Col10 & CD31 (brown). TF: tendon fibers; CD: calcium deposits, FM: fibrocartilaginous metaplasia; OM: osseous metaplasia; CT: connective tissue; V: vessels. Scale bar = 200 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31561454), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77461_rabbit-polyclonal-runx2-cbfa1-antibody-310202416154628.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0013.jpg "Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunocytochemistry-Immunofluorescence-H00000860-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunohistochemistry-Paraffin-H00000860-M01-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0001.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0012.jpg)
![Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Sandwich-ELISA-H00000860-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89104-img0012.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human tonsil shows moderate to strong nuclear positivity in a subset lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human rectum shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0009.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human salivary gland shows moderate to strong nuclear positivity in ductal cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RUNX2/CBFA1 Antibody [NBP1-89104] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Knockdown-Validated-NBP1-89104-img0014.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Western-Blot-H00000860-M06-img0001.jpg "Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunohistochemistry-Paraffin-H00000860-M06-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell . [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0003.jpg)
![ELISA: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-ELISA-H00000860-M06-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Western-Blot-NBP2-24755-img0002.jpg "Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.")
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0005.jpg "Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunocytochemistry-Immunofluorescence-NBP2-67777-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded mouse bone tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysateLane 2: U937 cell lysate Lane 3: HL-60 cell lysate](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0004.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0008.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-NBP1-77462-img0005.jpg "Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77462] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77462-img0006.jpg)
![Flow Cytometry: RUNX2/CBFA1 Antibody [NBP1-77462] - An intracellular stain was performed on HeLa cells with RUNX2/CBFA1 Antibody NBP1-77462AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Flow-Cytometry-NBP1-77462-img0007.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of RH-30 cells using the Anti-RUNX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunocytochemistry-Immunofluorescence-NBP2-52887-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells outside the reaction centrum.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human rectum shows nuclear positivty in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human small intestine shows nuclear immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human testis shows absence of staining (negative control).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Western-Blot-NBP2-52888-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Western-Blot-NBP2-52888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Staining of RH-30 cells using the Anti-RUNX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Immunocytochemistry-Immunofluorescence-NBP2-52888-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Immunohistochemistry-Paraffin-NBP2-52888-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Staining of human colon shows strong nuclear immunoreactivity in the lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Immunohistochemistry-Paraffin-NBP2-52888-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0235) [NBP2-52888] - Staining of human stomach shows strong nuclear immunoreactivity in the lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0235-Immunohistochemistry-Paraffin-NBP2-52888-img0002.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (7O7J4) [NBP3-15434] - Western blot analysis of extracts of various cell lines, using RUNX2/CBFA1 antibody (NBP3-15434) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-7O7J4-Western-Blot-NBP3-15434-img0002.jpg "Western Blot: RUNX2/CBFA1 Antibody (7O7J4) [NBP3-15434] - Western blot analysis of extracts of various cell lines, using RUNX2/CBFA1 antibody (NBP3-15434) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: RUNX2/CBFA1 Antibody (7O7J4) [NBP3-15434] - Western blot analysis of extracts of various cell lines, using RUNX2/CBFA1 antibody (NBP3-15434) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-7O7J4-Western-Blot-NBP3-15434-img0001.jpg)
![Immunoprecipitation: RUNX2/CBFA1 Antibody (7O7J4) [NBP3-15434] - Immunoprecipitation analysis of 300ug extracts of Mouse testis cells using 3ug RUNX2/CBFA1 antibody (NBP3-15434). Western blot was performed from the immunoprecipitate using RUNX2/CBFA1 antibody (NBP3-15434) at a dilition of 1:500.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-7O7J4-Immunoprecipitation-NBP3-15434-img0003.jpg)