SARS-CoV-2 Spike S1 Antibodies
The SARS-CoV-2 Spike (S) protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19. The SARS-CoV-2 Spike protein has a theoretical molecular weight of approximately 180 kDa and consists of a S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail. The viral Spike protein binds to the cell surface receptor angiotensin converting enzyme 2 (ACE2) resulting in fusion and virus entry into the cell. We have a catalog of SARS-CoV-2 Spike S1 monoclonal antibodies including human monoclonal, mouse monoclonal, rabbit monoclonal, and fluorescently-conjugated 2019-nCoV Spike S1 antibodies. Validated research applications for SARS-CoV-2 Spike S1 antibodies include Block/Neutralize, ELISA, Flow Cytometry, ICC, IHC, Immunofluorescence, and Western Blot. Browse the technical details, images, and related products to help you choose the viral-specific anti-SARS-CoV-2 Spike S1 antibody that is best suited for your research needs. Each SARS-CoV-2 S1 antibody is fully covered by Novus’ Guarantee+.
Alternate Names for SARS-CoV-2 Spike S1 Antibodies
anti-SARS-CoV-2 antibody, anti-Spike S1 Subunit antibody
9 Results for "sars-cov-2-spike-s1" in Primary Antibodies
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161943.jpg "Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161944.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike S1 antibody, NBP3-26918. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike S1 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161940.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in 293 Transfected Cells. Analysis of 4% paraformaldehyde-fixed Spike transfected 293 cells labeling SARS-CoV-2 Spike S1 with NBP3-26918 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161942.jpg)
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161941.jpg)
![Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, NBP3-07065 and NBP3-26919. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein, and the anti-SARS-CoV-2 Spike S1 antibody (NBP3-26919, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S1-Antibody-SP185-Sandwich-ELISA-NBP3-07065-img0001.jpg "Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, NBP3-07065 and NBP3-26919. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein, and the anti-SARS-CoV-2 Spike S1 antibody (NBP3-26919, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng.")
![Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, a Spike S1 recombinant protein as the binding protein, and an anti-SARS-CoV-2 Spike S1 antibody (1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S1-Antibody-SP185-Sandwich-ELISA-NBP3-07065-img0002.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] - Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike antibody (3 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07065_rabbit-monoclonal-sars-cov-2-spike-s1-antibody-sp185-2711202419293820.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] - Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 0.1 ug/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Spike S1 antibody cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7).](http://images.novusbio.com/fullsize/nbp3-07065_rabbit-monoclonal-sars-cov-2-spike-s1-antibody-sp185-271120241937754.jpg)
![Western Blot: SARS-CoV-2 Spike S1 Antibody [NBP3-20226] - Anti SARS-CoV-2 spike protein S1 domain staining of recombinant S1 protein (1 ug/ml). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NBP3-20226_Western-Blot-SARS-CoV-2-Spike-S1-Antibody-NBP3-20226-Anti-SARS-CoV-2-spike-protein-S1-domain-staining-of-recombinant-S1-protein-1-ug-ml-Detected-by-chemiluminescence--5420231130451.jpg "Western Blot: SARS-CoV-2 Spike S1 Antibody [NBP3-20226] - Anti SARS-CoV-2 spike protein S1 domain staining of recombinant S1 protein (1 ug/ml). Detected by chemiluminescence.")
![Western Blot: SARS-CoV-2 Spike S1 Antibody (H4) [NBP3-07032] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis](http://images.novusbio.com/fullsize/antibody/SARS-CoV-2-Spike-S1-Antibody-H4-Western-Blot-NBP3-07032-img0001.jpg "Western Blot: SARS-CoV-2 Spike S1 Antibody (H4) [NBP3-07032] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis")
![Western Blot: SARS-CoV-2 Spike S1 Antibody (B38) [NBP3-07031] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis](http://images.novusbio.com/fullsize/antibody/SARS-CoV-2-Spike-S1-Antibody-B38-Western-Blot-NBP3-07031-img0001.jpg "Western Blot: SARS-CoV-2 Spike S1 Antibody (B38) [NBP3-07031] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis")