14-3-3 Antibody (SR1456) - BSA Free

Western Blot: 14-3-3 Antibody (SR1456) [NBP3-21695] - Western blot analysis using NBP3-21695 in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: SR1456
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

14-3-3 Antibody (SR1456) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human 14-3-3 (Uniprot #: P31946, P63104)
• Specificity: Detects endogenous levels of 14-3-3
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YWHAQ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for 14-3-3 Antibody (SR1456) - BSA Free

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433
• 14-3-3
• 14-3-3,1C5
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAQ