Rabbit IgG Isotype Control

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Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ChIP, Flow, Func, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Protocols & FAQs
Support & Research

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 23034403)
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 32784470)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (PMID 255994242)
  • In vitro assay
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. In vitro assay reported in multiple scientific literatures with the Azide free version (NBP2-24893)
Reviewed Applications
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using
NBP2-24891 in the following applications:

Publications
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NBP2-24891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rabbit IgG Isotype Control

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Recent Reviews

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1
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Ranveer Singh
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol IGHG1