Rabbit IgG Isotype Control Summary
Description |
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
IGHG1 |
Purity |
Protein G purified |
Applications/Dilutions
Dilutions |
- Chromatin Immunoprecipitation (ChIP) 1:10-1:500
- Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 23034403)
- Flow Cytometry 1:10 - 1:1000
- Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 32784470)
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (PMID 255994242)
- In vitro assay
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Application Notes |
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. In vitro assay reported in multiple scientific literatures with the Azide free version (NBP2-24893) |
Reviewed Applications |
Read 1 Review rated 5 using NBP2-24891 in the following applications:
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Publications |
Read Publications using NBP2-24891 in the following applications:
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Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for Rabbit IgG Isotype Control
Background
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are
guaranteed for 1 year from date of receipt.
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